Construct: ORF TRCN0000478462
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005272.1_s317c1
- Derived from:
- ccsbBroadEn_11581
- DNA Barcode:
- GCAATGTCTACGCCGATCTTTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIAS (11019)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478462
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11019 | LIAS | lipoic acid synthetase | NM_001278591.1 | 100% | 100% | |
| 2 | human | 11019 | LIAS | lipoic acid synthetase | NM_001278592.1 | 70.4% | 46.6% | 218_219ins94;300_301ins32 |
| 3 | human | 11019 | LIAS | lipoic acid synthetase | NM_194451.3 | 42.9% | 41.6% | (many diffs) |
| 4 | human | 11019 | LIAS | lipoic acid synthetase | NM_001278590.2 | 42% | 40.7% | (many diffs) |
| 5 | human | 11019 | LIAS | lipoic acid synthetase | NM_006859.4 | 37.1% | 36% | (many diffs) |
| 6 | human | 11019 | LIAS | lipoic acid synthetase | NM_001363700.2 | 35% | 30.9% | (many diffs) |
| 7 | mouse | 79464 | Lias | lipoic acid synthetase | XM_006504195.2 | 61.2% | 42.7% | (many diffs) |
| 8 | mouse | 79464 | Lias | lipoic acid synthetase | NM_024471.5 | 33.1% | 31.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 492
- ORF length:
- 426
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tctacgctgc gggGATGCAG CCCGCACCCT GGGGCCCCGG GTATTTGGGA 121 GATATTTTTG CAGCCCAGTC AGACCGTTAA GCTCCTTGCC AGATAAAAAA AAGGAACTCC 181 TACAGAATGG ACCAGACCTT CAAGATTTTG TATCTGGTGA TCTTGCAGAC AGGAGCACCT 241 GGGATGAATA TAAAGGAAAC CTAAAACGCC AGAAAGGAGA AAGGTTAAGA CTACCTCCAT 301 GGCTAAAGAC AGAGATTCCC ATGGGGAAAA ATTACAATAA ACTGAAAAAT ACTTTGCGGA 361 ATTTAAATCT CCATACAGTA TGTGAGGAAG CTCGATGTCC CAATATTGGA GAGTGTTGGG 421 GAGGTGGAGA ATATGCCACC GCCACAGCCA CGATCATGGT AGGGCCAGCC TCAACCTCTA 481 TGGCTTTAGT CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCA ATGTCTACGC CGATCTTTCA 661 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t