Transcript: Mouse XM_006504195.2

PREDICTED: Mus musculus lipoic acid synthetase (Lias), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lias (79464)
Length:
407
CDS:
108..407

Additional Resources:

NCBI RefSeq record:
XM_006504195.2
NBCI Gene record:
Lias (79464)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006504195.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000076300 TCACGGTTAGAGCATTAAGTT pLKO.1 172 CDS 100% 5.625 4.500 N Lias n/a
2 TRCN0000076299 CGGTTAGAGCATTAAGTTCTT pLKO.1 175 CDS 100% 4.950 3.960 N Lias n/a
3 TRCN0000318298 CGGTTAGAGCATTAAGTTCTT pLKO_005 175 CDS 100% 4.950 3.960 N Lias n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006504195.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11581 pDONR223 100% 61.2% 42.7% None (many diffs) n/a
2 ccsbBroad304_11581 pLX_304 0% 61.2% 42.7% V5 (many diffs) n/a
3 TRCN0000478462 GCAATGTCTACGCCGATCTTTCAA pLX_317 93.9% 61.2% 42.7% V5 (many diffs) n/a
4 ccsbBroadEn_07727 pDONR223 100% 23.2% 17% None (many diffs) n/a
5 ccsbBroad304_07727 pLX_304 0% 23.2% 17% V5 (many diffs) n/a
6 TRCN0000474901 CTTTTTATTAACAATCCGAAGTAA pLX_317 42.6% 23.2% 17% V5 (many diffs) n/a
Download CSV