Construct: ORF TRCN0000478474
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011225.1_s317c1
- Derived from:
- ccsbBroadEn_14352
- DNA Barcode:
- AGCATCACGACGAATGGGCCACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEX261 (113419)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478474
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113419 | TEX261 | testis expressed 261 | NM_144582.3 | 75.8% | 75.5% | 1_141del;578A>C |
| 2 | mouse | 21766 | Tex261 | testis expressed gene 261 | XM_006505886.2 | 91.7% | 99.3% | (many diffs) |
| 3 | mouse | 21766 | Tex261 | testis expressed gene 261 | NM_009357.2 | 69.7% | 75.5% | (many diffs) |
| 4 | mouse | 21766 | Tex261 | testis expressed gene 261 | XM_006505888.3 | 43.5% | 41.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 513
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ctggttctcc accgctgtac tgattggcct ctacgtcttt gagcgcttcc 121 ccaccagcat gattggagtg ggcctattca ccaacctcgt ctactttggc ctccTCCAGA 181 CCTTCCCCTT CATCATGCTG ACCTCGCCTA ACTTCATCCT GTCGTGTGGA CTAGTGGTGG 241 TGAATCATTA CCTAGCATTT CAGTTTTTTG CAGAAGAATA TTATCCCTTC TCAGAGGTCC 301 TGGCCTATTT CACTTTCTGC CTGTGGATAA TTCCGTTTGC GTTTTTTGTG TCACTTTCGG 361 CCGGGGAGAA CGTCCTGCCC TCTACCATGC AGCCAGGAGA TGATGTCGTC TCCAATTATT 421 TCACCAAAGG CAAGCGGGGC AAACGCTTAG GGATCCTGGT TGTCTTCTCC TTCATCAAAG 481 AGGCCATTCT ACCCAGTCGT CCGAAGATAT ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 661 CATCACGACG AATGGGCCAC TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt