Construct: ORF TRCN0000478481

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000567.1_s317c1
Derived from:
ccsbBroadEn_07778
DNA Barcode:
TTTCATACATCCCTGAGACGTCTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEC63 (11231)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478481
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11231 SEC63 SEC63 homolog, protein tran... NM_007214.5 99.9% 100% 1251A>G
2 human 11231 SEC63 SEC63 homolog, protein tran... XM_017010218.2 51.7% 51.8% 0_1ins1098;153A>G
3 mouse 140740 Sec63 SEC63-like (S. cerevisiae) NM_153055.3 91.4% 96.3% (many diffs)
4 mouse 140740 Sec63 SEC63-like (S. cerevisiae) XM_011243116.1 91.3% 96.1% (many diffs)
5 mouse 140740 Sec63 SEC63-like (S. cerevisiae) XM_017313800.1 74.1% 77.8% (many diffs)
6 mouse 140740 Sec63 SEC63-like (S. cerevisiae) XM_017313801.1 46.2% 48.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2346
ORF length:
2280
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cgggcagcag ttccagtacg atgacagtgg gaacaccttc ttctacttcc
121tcacctcctt cgtggggctc atcgtgatcc cggcgacata ctacctctgg ccccgagatc
181agaatgccga gcaaattcga ttaaagaata tcagaaaagt atatggaagg tgtatgtggt
241atcgtttacg gttattaaaa ccccagccaa atattattcc tacagtaaag aaaatagttc
301tgcttgcagg atgggcattg ttcttattcc ttgcatataa agtttccaaa acagaccgag
361aataccaaga atacaatcct tatgaagtat taaatttgga tcctggagcc acagtagcag
421aaattaaaaa acaatatcgt ttgctgtcac ttaaatatca tccagataaa ggaggtgatg
481aggttatgtt catgaggata gcaaaagctt atgctgcttt aacggatgaa gagtcccgga
541aaaattggga agaatttgga aatccagatg ggcctcaagc cacaagcttt ggaattgccc
601tgccagcttg gatagttgac cagaaaaact caattctggt tttacttgta tatggattgg
661catttatggt tatccttcca gttgttgtgg gctcttggtg gtatcgctca atacgctata
721gtggagacca gattctaata cgcacaacac agatttatac atactttgtt tataaaaccc
781gaaatatgga tatgaaacgt cttatcatgg ttttggctgg agcttctgaa tttgatcctc
841agtataataa agatgccaca agcagaccaa cggataatat tctaatacca cagctaatca
901gagaaattgg cagcattaat ttaaagaaga atgagcctcc acttacctgc ccatatagcc
961tgaaggccag agttctttta ctgtctcatc ttgctagaat gaaaattcct gagacccttg
1021aagaagatca gcaattcatg ctaaaaaagt gtcctgccct acttcaagaa atggttaatg
1081taatctgcca actaatagta atggcccgga accgtgaaga aagggagttt cgtgctccaa
1141ctttggcatc cctagaaaac tgcatgaagc tttctcagat ggccgttcag ggacttcagc
1201aatttaagtc tccccttctg cagctccctc atattgaaga ggacaatctt agacgggttt
1261ctaatcataa gaagtataaa attaaaacta tccaggattt ggtgagttta aaagagtcag
1321atcgtcacac tctactgcac ttccttgaag atgaaaaata tgaagaggtt atggctgtcc
1381ttgggagttt tccatatgtg accatggata taaaatcaca ggtgttagat gatgaagata
1441gcaacaacat cacagtagga tccttagtta cagtgttggt taagttgaca aggcaaacaa
1501tggctgaagt atttgaaaag gagcagtcca tctgtgctgc agaggaacag ccagcagaag
1561atgggcaggg tgaaactaac aagaacagga caaaaggagg atggcaacag aagagtaaag
1621gacccaagaa aactgctaaa tcaaaaaaaa agaaaccttt aaaaaaaaaa cctacacctg
1681tgctattacc acagtcaaag caacagaaac aaaagcaggc aaatggagtc gttgggaatg
1741aagctgcagt aaaggaagat gaagaagaag tttcagataa gggcagtgat tctgaagaag
1801aagaaaccaa tagagattcc caaagtgaga aagatgatgg tagtgacaga gactctgata
1861gagagcaaga tgaaaaacaa aacaaagatg atgaagcaga gtggcaagaa ttacaacaaa
1921gcatacagcg aaaagagaga gctctattgg aaaccaaatc aaaaataaca catcctgtgt
1981atagccttta ctttccTGAG GAAAAACAAG AATGGTGGTG GCTTTACATT GCAGATAGGA
2041AGGAGCAGAC ATTAATATCC ATGCCATATC ATGTGTGTAC GCTGAAAGAT ACAGAGGAGG
2101TAGAGCTGAA GTTTCCTGCA CCAGGCAAGC CTGGAAATTA TCAGTATACT GTGTTTCTGA
2161GATCAGACTC CTATATGGGT TTGGATCAGA TTAAACCATT GAAGTTGGAA GTTCATGAGG
2221CTAAGCCTGT GCCAGAAAAT CACCCACAGT GGGATACAGC AATAGAGGGG GATGAAGACC
2281AGGAGGACAG TGAGGGCTTT GAAGATAGCT TTGAGGAAGA AGAGGAGGAA GAAGAAGATG
2341ATGACTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
2401TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
2461CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTTCATACA TCCCTGAGAC GTCTAACGCG
2521TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)