Construct: ORF TRCN0000478528
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007867.1_s317c1
- Derived from:
- ccsbBroadEn_03796
- DNA Barcode:
- GACATTGCCCATTAGATCCATACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMIM8 (57150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478528
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57150 | SMIM8 | small integral membrane pro... | NM_001042493.3 | 100% | 100% | |
| 2 | human | 57150 | SMIM8 | small integral membrane pro... | NM_020425.5 | 100% | 100% | |
| 3 | human | 57150 | SMIM8 | small integral membrane pro... | NM_001287445.2 | 64.4% | 43.8% | (many diffs) |
| 4 | mouse | 66291 | Smim8 | small integral membrane pro... | NM_001330755.1 | 85.5% | 88.6% | (many diffs) |
| 5 | mouse | 66291 | Smim8 | small integral membrane pro... | NM_001330756.1 | 85.5% | 88.6% | (many diffs) |
| 6 | mouse | 66291 | Smim8 | small integral membrane pro... | NM_001330757.1 | 85.5% | 88.6% | (many diffs) |
| 7 | mouse | 66291 | Smim8 | small integral membrane pro... | NM_025471.3 | 85.5% | 88.6% | (many diffs) |
| 8 | mouse | 66291 | Smim8 | small integral membrane pro... | XM_006538163.2 | 85.5% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 381
- ORF length:
- 291
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCTTCAGC ACCTGAGCCT CCAACATTCA 121 AAAAGGAACC ACCCAAAGAG AAAGAGTTTC AAAGCCCAGG GCTCAGAGGG GTGCGCACAA 181 CAACCTTATT TCGTGCTGTG AATCCAGAGC TCTTCATTAA ACCTAACAAA CCTGTAATGG 241 CTTTCGGATT GGTAACTCTT TCACTTTGCG TGGCATATAT TGGTTATCTA CATGCAATAC 301 AAGAGAATAA AAAGGACCTC TATGAAGCTA TTGATAGTGA GGGGCACAGT TATATGAGGC 361 GGAAAACATC CAAATGGGAT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGACA TTGCCCATTA 541 GATCCATACG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt