Construct: ORF TRCN0000478532
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000133.1_s317c1
- Derived from:
- ccsbBroadEn_04737
- DNA Barcode:
- AGCCAATTATCCTGTTGTGTCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANAPC16 (119504)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478532
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 119504 | ANAPC16 | anaphase promoting complex ... | NM_001242546.1 | 100% | 100% | |
| 2 | human | 119504 | ANAPC16 | anaphase promoting complex ... | NM_001242547.2 | 100% | 100% | |
| 3 | human | 119504 | ANAPC16 | anaphase promoting complex ... | NM_001366791.1 | 100% | 100% | |
| 4 | human | 119504 | ANAPC16 | anaphase promoting complex ... | NM_173473.4 | 100% | 100% | |
| 5 | human | 119504 | ANAPC16 | anaphase promoting complex ... | NM_001242548.1 | 77.2% | 77.2% | 141_142ins75 |
| 6 | human | 119504 | ANAPC16 | anaphase promoting complex ... | NR_038392.2 | 7.8% | (many diffs) | |
| 7 | human | 119504 | ANAPC16 | anaphase promoting complex ... | NR_038391.2 | 7.4% | (many diffs) | |
| 8 | mouse | 52717 | Anapc16 | anaphase promoting complex ... | NM_025514.3 | 90% | 100% | (many diffs) |
| 9 | mouse | 52717 | Anapc16 | anaphase promoting complex ... | NM_001347451.1 | 76.7% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 396
- ORF length:
- 330
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcttcatca tcatcctccT CAGCTGGTGG GGTCAGTGGA AGTTCTGTCA 121 CTGGATCTGG TTTCAGTGTC TCAGACCTTG CCCCACCACG GAAAGCCCTT TTCACCTACC 181 CCAAAGGAGC TGGAGAGATG TTAGAAGATG GCTCTGAGAG ATTCCTCTGC GAATCTGTTT 241 TTAGCTATCA AGTGGCATCC ACGCTTAAAC AGGTGAAACA TGATCAGCAA GTTGCTCGGA 301 TGGAAAAACT AGCTGGTTTG GTAGAAGAGC TGGAGGCTGA CGAGTGGCGG TTTAAGCCCA 361 TCGAGCAGCT GCTGGGATTC ACCCCCTCTT CAGGTTGCCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 AAGCCAATTA TCCTGTTGTG TCCCGACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt