Construct: ORF TRCN0000478535
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013827.1_s317c1
- Derived from:
- ccsbBroadEn_01571
- DNA Barcode:
- GGTGTACGTTAGAAACCGGCCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNRPD2 (6633)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478535
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6633 | SNRPD2 | small nuclear ribonucleopro... | NM_004597.6 | 100% | 100% | |
| 2 | human | 6633 | SNRPD2 | small nuclear ribonucleopro... | NM_001369751.1 | 91.5% | 91.5% | 0_1ins30 |
| 3 | human | 6633 | SNRPD2 | small nuclear ribonucleopro... | NM_177542.3 | 91.5% | 91.5% | 0_1ins30 |
| 4 | human | 6633 | SNRPD2 | small nuclear ribonucleopro... | NM_001369752.1 | 54.7% | 45.5% | 181_206del;234_235ins146 |
| 5 | mouse | 107686 | Snrpd2 | small nuclear ribonucleopro... | NM_026943.1 | 91.2% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 420
- ORF length:
- 354
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ccTCCTCAAC AAGCCCAAGA GTGAGATGAC CCCAGAGGAG CTGCAGAAGC 121 GAGAGGAGGA GGAATTTAAC ACCGGTCCAC TCTCTGTGCT CACACAGTCA GTCAAGAACA 181 ATACCCAAGT GCTCATCAAC TGCCGCAACA ATAAGAAACT CCTGGGCCGC GTGAAGGCCT 241 TCGATAGGCA CTGCAACATG GTGCTGGAGA ACGTGAAGGA GATGTGGACT GAGGTACCCA 301 AGAGTGGCAA GGGCAAGAAG AAGTCCAAGC CAGTCAACAA AGACCGCTAC ATCTCCAAGA 361 TGTTCCTGCG CGGGGACTCA GTCATCGTGG TCCTGCGGAA CCCGCTCATC GCCGGCAAGT 421 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 481 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 541 TTTATATATC TTGTGGAAAG GACGAGGTGT ACGTTAGAAA CCGGCCGGGA CGCGTTAAGT 601 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt