Construct: ORF TRCN0000478551

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017953.2_s317c1
Derived from:
ccsbBroadEn_12628
DNA Barcode:
GTTTATTGTGAGATATCGAATTCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMC5 (79838)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478551
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79838 TMC5 transmembrane channel like 5 NM_024780.5 85% 85% 1_339del;902T>C
2 human 79838 TMC5 transmembrane channel like 5 NM_001105248.1 64.2% 64.2% 1_1077del;1640T>C
3 human 79838 TMC5 transmembrane channel like 5 NM_001261841.2 64.2% 64.2% 1_1077del;1640T>C
4 human 79838 TMC5 transmembrane channel like 5 NM_001308161.1 59.1% 59% 1_1077del;1640T>C;1781_1782ins156
5 human 79838 TMC5 transmembrane channel like 5 NM_001105249.1 58.5% 58.4% 1_1077del;1640T>C;2573_2574ins174
6 mouse 74424 Tmc5 transmembrane channel-like ... NM_028930.3 72.1% 72.8% (many diffs)
7 mouse 74424 Tmc5 transmembrane channel-like ... XM_006508253.2 56.9% 57.4% (many diffs)
8 mouse 74424 Tmc5 transmembrane channel-like ... NM_001105252.1 56.5% 57% (many diffs)
9 mouse 74424 Tmc5 transmembrane channel-like ... XM_011241924.2 56.5% 57% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2007
ORF length:
1941
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac atccagagac agaattaaag ccatcaggaa ccagccaagg accatggaag
121agaaaaggaa ccttaggaaa atagttgaca aagaaaaaag caaacagacc catcgtatcc
181ttcagctcaa ttgctgtatt cagtgtctga actccatttc ccgggcttat cggagatcca
241agaacagcct gtcggaaatt ctgaattcca tcagcctgtg gcagaagacg ctgaagatca
301ttggaggcaa gtttggaacc agcgtcctct cctatttcaa ctttctgaga tggcttttga
361agttcaacat tttctcattc atcctgaact tcagcttcat cataatccct cagtttaccg
421tggccaaaaa gaacaccctc cagttcactg ggctggagtt tttcactggg gtgggttatt
481ttagggacac agtgatgtac tatggctttt acaccaattc caccatccag cacgggaaca
541gcggggcatc ctacaacatg cagctggcct acatcttcac aatcggagca tgcttgacca
601cctgcttctt cagtttgctg ttcagcacgg ccaagtattt ccggaacaac ttcattaatc
661cccacattta ctccggaggg atcaccaagc tgatcttttg ctgggacttc actgtcactc
721atgaaaaagc tgtgaagcta aaacagaaga atcttagcac tgagataagg gagaacctgt
781cagagctccg tcaggagaat tccaagttga cgttcaatca gctgctgacc cgcttctctg
841cctacatggt agcctgggtt gtctctacag gagtggccat agcctgctgt gcagccgttt
901attacctggc tgagtacaac ttagagttcc tgaagacaca cagtaaccct ggggcggtgc
961tgttactgcc tttcgttgtg tcctgcatta atctggccgt gccatgcatc tactccatgt
1021tcaggcttgt ggagaggtac gagatgccac ggcacgaagt ctacgttctc ctgatccgaa
1081acatcttttt gaaaatatca atcattggca ttctttgtta ctattggctc aacaccgtgg
1141ccctgtctgg tgaagagtgt tgggaaaccc tcattggcca ggacatctac cggctccttc
1201tgatggattt tgtgttctct ttagtcaatt ccttcctggg ggagtttctg aggagaatca
1261ttgggatgca actgatcaca agtcttggcc ttcaggagtt tgacattgcc aggaacgttc
1321tagaactgat ctatgcacaa actctggtgt ggattggcat cttcttctgc cccctgctgc
1381cctttatcca aatgattatg cttttcatca tgttctactc caaaaatatc agcctgatga
1441tgaatttcca gcctccgagc aaagcctggc gggcctcaca gatgatgact ttcttcatct
1501tcttgctctt tttcccatcc ttcaccgggg tcttgtgcac cctggccatc accatctgga
1561gattgaagcc ttcagctgac tgtggccctt ttcgaggtct gcctctcttc attcactcca
1621tctacagctg gatcgacacc ctaagtacac ggcctggcta cctgtgggtt gtttggatct
1681atcggaacct cattggaagt gtgcacttct ttttcatcct caccctcatt gtgctaatca
1741tcacctatct ttactggcag atcacagagg gaaggaagat tatgataagg ctgctCCATG
1801AGCAGATCAT TAATGAGGGC AAAGATAAAA TGTTCCTGAT AGAAAAATTG ATCAAGCTGC
1861AGGATATGGA GAAGAAAGCA AACCCCAGCT CACTTGTTCT GGAAAGGAGA GAGGTGGAGC
1921AACAAGGCTT TTTGCATTTG GGGGAACATG ATGGCAGTCT TGACTTGCGA TCTAGAAGAT
1981CAGTTCAAGA AGGTAATCCA AGGGCCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG
2041GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2101AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTTTATTG
2161TGAGATATCG AATTCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2221aagatt

Download FASTA (ORF) (Full)