Construct: ORF TRCN0000478588

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011166.1_s317c1
Derived from:
ccsbBroadEn_08601
DNA Barcode:
GCATTTCCCTGGCCCAACACTCAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DBNDD2 (55861)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478588
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55861 DBNDD2 dysbindin domain containing 2 NM_001048221.2 99.7% 99.3% 280A>G
2 human 55861 DBNDD2 dysbindin domain containing 2 NM_001048223.2 99.7% 99.3% 280A>G
3 human 55861 DBNDD2 dysbindin domain containing 2 NM_001197139.1 99.7% 99.3% 280A>G
4 human 55861 DBNDD2 dysbindin domain containing 2 NM_001197140.1 99.7% 99.3% 280A>G
5 human 55861 DBNDD2 dysbindin domain containing 2 NM_001048222.2 64.4% 59.6% (many diffs)
6 human 55861 DBNDD2 dysbindin domain containing 2 NM_001048224.2 64.4% 59.6% (many diffs)
7 human 55861 DBNDD2 dysbindin domain containing 2 NM_018478.3 62% 61.7% 1_294del;574A>G
8 human 55861 DBNDD2 dysbindin domain containing 2 NM_001048225.2 61% 60.8% 1_306del;586A>G
9 human 55861 DBNDD2 dysbindin domain containing 2 NM_001048226.2 39.6% 36.5% (many diffs)
10 human 767557 SYS1-DBNDD2 SYS1-DBNDD2 readthrough (NM... NR_003189.2 35.8% 1_388del;668A>G;872_1344del
11 mouse 52840 Dbndd2 dysbindin (dystrobrevin bin... NM_001048227.1 84% 84.4% (many diffs)
12 mouse 52840 Dbndd2 dysbindin (dystrobrevin bin... NM_001048228.1 84% 84.4% (many diffs)
13 mouse 52840 Dbndd2 dysbindin (dystrobrevin bin... NM_001048229.1 84% 84.4% (many diffs)
14 mouse 52840 Dbndd2 dysbindin (dystrobrevin bin... NM_026797.2 84% 84.4% (many diffs)
15 mouse 52840 Dbndd2 dysbindin (dystrobrevin bin... XM_006499895.3 84% 84.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
549
ORF length:
483
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga cccaaatcct cgggccgccc tggagcgcca gcagctccgc cttcgggagc
121ggcaaaaatt cttcgaggac attttacagc cagagacaga gtttgtcttt ccTCTGTCCC
181ATCTGCATCT CGAGTCGCAG AGACCCCCCA TAGGTAGTAT CTCATCCATG GAAGTGAATG
241TGGACACACT GGAGCAAGTA GAACTTATTG ACCTTGGGGA CCCGGATGCA GCAGATGTGT
301TCTTGCCTTG CGAAGATCCT CCACCAACCC CCCAGTCGTC TGGGGTGGAC AACCATTTGG
361AGGAGCTGAG CCTGCCGGTG CCTACATCAG ACAGGACCAC ATCTAGGACC TCCTCCTCCT
421CCTCCTCCGA CTCCTCCACC AACCTGCATA GCCCAAATCC AAGTGATGAT GGAGCAGATA
481CGCCCTTGGC ACAGTCGGAT GAAGAGGAGG AAAGGGGTGA TGGAGGGGCA GAGCCTGGAG
541CCTGCAGCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
601CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
661TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCATTT CCCTGGCCCA ACACTCAGAC
721GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)