Construct: ORF TRCN0000478590
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009951.1_s317c1
- Derived from:
- ccsbBroadEn_08745
- DNA Barcode:
- GCCGAACCTAACTACTTGTCAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDH10 (57575)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478590
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57575 | PCDH10 | protocadherin 10 | NM_032961.3 | 99.9% | 99.9% | 1214T>C |
| 2 | human | 57575 | PCDH10 | protocadherin 10 | XM_011532150.2 | 98.1% | 97.8% | (many diffs) |
| 3 | human | 57575 | PCDH10 | protocadherin 10 | NM_020815.3 | 85.3% | 84.6% | (many diffs) |
| 4 | mouse | 18526 | Pcdh10 | protocadherin 10 | NM_001098171.1 | 89.7% | 97.3% | (many diffs) |
| 5 | mouse | 18526 | Pcdh10 | protocadherin 10 | NM_001098172.1 | 88.5% | 96.1% | (many diffs) |
| 6 | mouse | 18526 | Pcdh10 | protocadherin 10 | NM_001098170.1 | 87.8% | 95% | (many diffs) |
| 7 | mouse | 18526 | Pcdh10 | protocadherin 10 | NM_011043.3 | 75.8% | 82% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3189
- ORF length:
- 3120
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gattgtgcta ttattgtttg ccttgctctg gatggtggaa ggagtctttt 121 cccagcttca ctacacggta caggaggagc aggaacatgg cactttcgtg gggaatatcg 181 ctgaagatct gggtctggac attacaaaac tttcggctcg cgggtttcag acggtgccca 241 actcaaggac cccttactta gacctcaacc tggagacagg ggtgctgtac gtgaacgaga 301 aaatagaccg cgaacaaatc tgcaaacaga gcccctcctg tgtcctgcac ctggaggtct 361 ttctggagaa ccccctggag ctgttccagg tggagatcga ggtgctggac attaatgaca 421 accccccctc tttcccggag ccagacctga cggtggaaat ctctgagagc gccacgccag 481 gcactcgctt ccccttggag agcgcattcg acccagacgt gggcaccaac tccttgcgcg 541 actacgagat cacccccaac agctacttct ccctggacgt gcagacccag ggggatggca 601 accgattcgc tgagctggtg ctggagaagc cactggaccg agagcagcaa gcggtgcacc 661 gctacgtgct gaccgcggtg gacggaggag gtgggggagg agtaggagaa ggagggggag 721 gtggcggggg agcaggcctg cccccccagc agcagcgcac cggcacggcc ctactcacca 781 tccgagtgct ggactccaat gacaatgtgc ccgctttcga ccaacccgtc tacactgtgt 841 ccctaccaga gaactctccc ccaggcactc tcgtgatcca gctcaacgcc accgacccgg 901 acgagggcca gaacggtgag gtcgtgtact ccttcagcag ccacatttcg ccccgggcgc 961 gggagctttt cggactctcg ccgcgcactg gcagactgga ggtaagcggc gagttggact 1021 atgaagagag cccagtgtac caagtgtacg tgcaagccaa ggacctgggc cccaacgccg 1081 tgcctgcgca ctgcaaggtg ctagtgcgag tactggatgc taatgacaac gcgccagaga 1141 tcagcttcag caccgtgaag gaagcggtga gtgagggcgc ggcgcccggc actgtggtgg 1201 cccttttcag cgtgactgac cgcgactcag aggagaatgg gcaggtgcag tgcgagctac 1261 tgggagacgt gcctttccgc cccaagtctt cctttaagaa ttactacacc atcgttaccg 1321 aagcccccct ggaccgagag gcgggggact cctacaccct gactgtagtg gctcgggacc 1381 ggggcgagcc tgcgctctcc accagtaagt cgatccaggt acaagtgtcg gatgtgaacg 1441 acaacgcgcc gcgtttcagc cagccggtct acgacgtgta tgtgactgaa aacaacgtgc 1501 ctggcgccta catctacgcg gtgagcgcca ccgaccggga tgagggcgcc aacgcccagc 1561 ttgcctactc tatcctcgag tgccagatcc agggcatgag cgtcttcacc tacgtttcta 1621 tcaactctga gaacggctac ttgtacgccc tgcgctcctt cgactatgag cagctgaagg 1681 acttcagttt tcaggtggaa gcccgggacg ctggcagccc ccaggcgctg gctggtaacg 1741 ccactgtcaa catcctcata gtggatcaaa atgacaacgc ccctgccatc gtggcgcctc 1801 taccagggcg caacgggact ccagcgcgtg aggtgctgcc ccgctcggcg gagccgggtt 1861 acctgctcac ccgcgtggcc gccgtggacg cggacgacgg cgagaacgcc cggctcactt 1921 acagcatcgt gcgtggcaac gaaatgaacc tctttcgcat ggactggcgc accggggagc 1981 tgcgcacagc acgccgagtc ccggccaagc gcgaccccca gcggccttat gagctggtga 2041 tcgaggtgcg cgaccatggg cagccgcccc tttcctccac cgccaccctg gtggttcagc 2101 tggtggatgg cgccgtggag ccccagggcg ggggcgggag cggaggcgga gggtcaggag 2161 agcaccagcg ccccagtcgc tctggcggcg gggaaacctc gctagacctc accctcatcc 2221 tcatcatcgc gttgggctcg gtgtccttca tcttcctgct ggccatgatc gtgctggccg 2281 tgcgttgcca aaaagagaag aagctcaaca tctatacttg tctggccagc gattgctgcc 2341 tctgctgctg ctgctgcggt ggcggaggtt cgacctgctg tggccgccaa gcccgggcgc 2401 gcaagaagaa actcagcaag tcagacatca tgctggtgca gagctccaat gtacccagta 2461 acccggccca ggtgccgata gaggagtccg ggggctttgg ctcccaccac cacaaccaga 2521 attactgcta tcaggtatgc ctgacccctg agtccgccaa gaccgacctg atgtttctta 2581 agccctgcag cccttcgcgg agtacggaca ctgagcacaa cccctgcggg gccatcgtca 2641 ccggttacac cgaccagcag cctgatatca tctccaacgg aagcattttg tccaacgaga 2701 ctaaacacca gcgagcagag ctcagctatc tagttgacag acctcgccga gttaacagtt 2761 ctgcattcca ggaagccgac atagtaagct ctaaggacag tggtcatgga gacagtgaac 2821 agggagatag tgatcatgat gccaccaacc gtgcccagtc agctggtatg gaTCTCTTCT 2881 CCAATTGCAC TGAGGAATGT AAAGCTCTGG GCCACTCAGA TCGGTGCTGG ATGCCTTCTT 2941 TTGTCCCTTC TGATGGACGC CAGGCTGCTG ATTATCGCAG CAATCTGCAT GTTCCTGGCA 3001 TGGACTCTGT TCCAGACACT GAGGTGTTTG AAACTCCAGA AGCCCAGCCT GGGGCAGAGC 3061 GGTCCTTTTC CACCTTTGGC AAAGAGAAGG CCCTTCACAG CACTCTGGAG AGGAAGGAGC 3121 TGGATGGACT GCTGACTAAT ACGCGAGCGC CTTACAAACC ACCATATTTG ACACGGAAAA 3181 GGATATGCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3241 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3301 TTTCTTGGCT TTATATATCT TGGGAAAGGA CGAGCCGAAC CTAACTACTT GTCAGTTACG 3361 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt