Construct: ORF TRCN0000478596
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015523.2_s317c1
- Derived from:
- ccsbBroadEn_09936
- DNA Barcode:
- TTCTCAGGCTTTGGCAACTATCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PGM2L1 (283209)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478596
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283209 | PGM2L1 | phosphoglucomutase 2 like 1 | NM_173582.6 | 99.8% | 99.6% | 41T>C;1591G>A;1710T>C |
| 2 | human | 283209 | PGM2L1 | phosphoglucomutase 2 like 1 | XM_011544953.3 | 96.5% | 96.4% | (many diffs) |
| 3 | mouse | 70974 | Pgm2l1 | phosphoglucomutase 2-like 1 | NM_027629.3 | 89.8% | 93.2% | (many diffs) |
| 4 | mouse | 70974 | Pgm2l1 | phosphoglucomutase 2-like 1 | XM_006508204.1 | 89.8% | 93.2% | (many diffs) |
| 5 | mouse | 70974 | Pgm2l1 | phosphoglucomutase 2-like 1 | XM_006508205.1 | 89.8% | 93.2% | (many diffs) |
| 6 | mouse | 70974 | Pgm2l1 | phosphoglucomutase 2-like 1 | XR_378271.1 | 38.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1932
- ORF length:
- 1866
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaaaacaca gagggggatc tgaactccaa cctgccccac gccccctacc 121 acaccgggga ccctcagctg gacacggcca tcgggcagtg gctccgctgg gataagaatc 181 ccaaaacaaa agagcagatt gaaaacctgt tacggaatgg gatgaacaag gagctgcgag 241 atcgtctttg ttgccgaatg acttttggga ctgcaggact tcgttctgcc atgggggcag 301 ggttttgcta tattaatgac cttacagtaa tacagtcaac acaggggatg tacaaatacc 361 ttgagagatg tttctcagac ttcaagcaga gaggctttgt ggttgggtat gacactcggg 421 gtcaagtaac tagcagctgc agcagccaga ggcttgctaa actcactgct gcagtcttgc 481 tggccaaaga tgttcctgtg tacctttttt caagatatgt tcctacacct tttgtaccat 541 atgcagttca gaagctcaaa gcagttgcag gtgtgatgat tactgcctct cacaaccgca 601 aggaagacaa tggatacaag gtttactggg aaactggtgc tcagatcaca tctcctcatg 661 ataaagaaat tctaaaatgt atagaagaat gtgtggaacc ctggaatggt tcctggaatg 721 ataatttagt ggataccagc ccgctgaaga gagaccctct gcaggacatt tgcaggagat 781 acatggaaga tctgaaaaag atctgttttt acagggagtt aaactcgaag accaccttga 841 aatttgtgca cacatctttt catggggtcg gacatgacta tgtgcagttg gcttttaaag 901 tgtttggttt taagcctcca attccagtac cagaacaaaa agatcctgat ccagactttt 961 ctaccgttaa atgtccaaat cctgaagaag gagaatctgt gctggaactt tccttgagac 1021 tggcagagaa agaaaatgcc cgggtagtgc tagccacaga tcctgatgca gacagactgg 1081 cagcagcaga acttcaggag aatggttgtt ggaaagtttt cacagggaat gagttggcag 1141 ctttgtttgg atggtggatg tttgattgct ggaagaaaaa taaatcaaga aatgctgatg 1201 tgaagaacgt ttatatgtta gccaccacag tctcttctaa aattctgaag gcaattgcac 1261 ttaaagaagg atttcatttt gaagaaacat taccaggttt taaatggatt ggaagtagga 1321 taatagacct cctggaaaat gggaaagaag tcctttttgc atttgaagag tctattggtt 1381 ttctctgtgg aacttcagtt ttggataaag atggggtgag tgcagctgtt gtggttgctg 1441 agatggcatc ttacctggaa accatgaata taacattgaa acagcaactg gttaaggttt 1501 atgaaaaata tggttatcat atttcaaaaa cttcctattt cttgtgttat gaaccaccta 1561 ccatcaaaag tatatttgaa aggcttcgta attttgattc tccaaaagaa tatccaaaat 1621 tttgtggaac attTGCTATA TTGCATGTAC GGGACATTAC CACTGGATAT GACAGTAGCC 1681 AGCCTAATAA GAAATCAGTG CTGCCTGTGA GTAAAAACAG CCAAATGATT ACATTTACTT 1741 TTCAAAATGG CTGTGTTGCT ACCCTTCGGA CAAGCGGAAC AGAACCAAAG ATAAAGTATT 1801 ATGCAGAGAT GTGTGCGTCA CCTGACCAGA GTGACACTGC TTTACTGGAG GAAGAACTGA 1861 AGAAACTCAT TGATGCTCTG ATAGAGAATT TTCTTCAGCC TAGTAAGAAT GGACTGATCT 1921 GGCGTTCTGT TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1981 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2041 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTC TCAGGCTTTG GCAACTATCT 2101 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t