Construct: ORF TRCN0000478606
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015438.1_s317c1
- Derived from:
- ccsbBroadEn_12762
- DNA Barcode:
- GCTGACTAAATAAGGCGCGCCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK40 (83931)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478606
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83931 | STK40 | serine/threonine kinase 40 | NM_001282547.2 | 53.4% | 53.3% | 1_606del;1183G>A |
| 2 | human | 83931 | STK40 | serine/threonine kinase 40 | NM_032017.3 | 53.4% | 53.3% | 1_606del;1183G>A |
| 3 | human | 83931 | STK40 | serine/threonine kinase 40 | NM_001282546.2 | 52.8% | 52.7% | 1_621del;1198G>A |
| 4 | mouse | 74178 | Stk40 | serine/threonine kinase 40 | NM_028800.3 | 48.3% | 52.1% | (many diffs) |
| 5 | mouse | 74178 | Stk40 | serine/threonine kinase 40 | XM_006503442.1 | 48.3% | 52.1% | (many diffs) |
| 6 | mouse | 74178 | Stk40 | serine/threonine kinase 40 | XM_017320414.1 | 48.3% | 52.1% | (many diffs) |
| 7 | mouse | 74178 | Stk40 | serine/threonine kinase 40 | NM_001145827.1 | 46.8% | 50.5% | (many diffs) |
| 8 | mouse | 74178 | Stk40 | serine/threonine kinase 40 | XM_006503441.3 | 46.8% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 765
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gctcaacaag aggacacatc ggataaccat caccaacttc tgcctcggga 121 agcatctggt gagcgagggg gacctgctga aggaccagag agggagccct gcctacatca 181 gtcccgacgt gctcagcggc cggccgtacc gtggcaagcc cagtgacatg tgggccctgg 241 gcgtggtgct cttcaccatg ctgtatggcc agttcccctt ctacgacagc atcccgcagg 301 agctcttccg caagatcaag gctgccgagt ataccattcc tgaggatgga cgggtttctg 361 agaacaccgt gtgtctcatc cggaagctgc tggtccttga cccccagcag cgcctggccg 421 ccgccgacgt cctggaggcc ctcagtgcca tcattgcatc atggcagtcc ctgtcatctc 481 TGAGTGGGCC TTTGCAAGTG GTTCCTGACA TTGATGACCA AATGAGCAAT GCGGATAGCT 541 CCCAGGAGGC GAAGGTGACG GAGGAGTGCT CCCAGTACGA GTTTGAGAAC TACATGCGTC 601 AGCAGCTGCT GCTGGCCGAG GAGAAGAGCT CCATCCATGA CACCCGGAGC TGGGTACCCA 661 AGCGGCAGTT CGGCAGCGCA CCACCGGTGC GACGGCTGGG CCACGACGCA CAGCCCATGA 721 CCTCCTTGGA CACGGCCATC CTGGCGCAGC GCTACCTGCG GAAATACCCA ACTTTCTTGT 781 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 841 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 901 GAAAGGACGA GCTGACTAAA TAAGGCGCGC CACGACGCGT TAAGTCgaca atcaacctct 961 ggattacaaa atttgtgaaa gatt