Construct: ORF TRCN0000478613
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003137.2_s317c1
- Derived from:
- ccsbBroadEn_06747
- DNA Barcode:
- CCCCCTATTCGAATCAAACGGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLD1 (5424)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478613
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5424 | POLD1 | DNA polymerase delta 1, cat... | NM_001256849.1 | 99.9% | 99.9% | 324G>A;356G>A |
| 2 | human | 5424 | POLD1 | DNA polymerase delta 1, cat... | NM_002691.4 | 99.9% | 99.9% | 324G>A;356G>A |
| 3 | human | 5424 | POLD1 | DNA polymerase delta 1, cat... | XM_011527038.1 | 99.9% | 99.9% | 324G>A;356G>A |
| 4 | human | 5424 | POLD1 | DNA polymerase delta 1, cat... | XM_017026881.1 | 99.9% | 99.9% | 324G>A;356G>A |
| 5 | human | 5424 | POLD1 | DNA polymerase delta 1, cat... | XM_005259008.4 | 97.7% | 97.7% | 324G>A;356G>A;2080_2081ins72 |
| 6 | human | 5424 | POLD1 | DNA polymerase delta 1, cat... | XM_017026882.2 | 97.7% | 97.7% | 324G>A;356G>A;2080_2081ins72 |
| 7 | human | 5424 | POLD1 | DNA polymerase delta 1, cat... | NM_001308632.1 | 97.6% | 97.6% | 324G>A;356G>A;1775_1852del |
| 8 | human | 5424 | POLD1 | DNA polymerase delta 1, cat... | NR_046402.2 | 93.5% | (many diffs) | |
| 9 | human | 5424 | POLD1 | DNA polymerase delta 1, cat... | XR_935835.2 | 63.8% | (many diffs) | |
| 10 | mouse | 18971 | Pold1 | polymerase (DNA directed), ... | NM_011131.3 | 84.4% | 89.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3387
- ORF length:
- 3321
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tggcaagcgg cggccaggcc cagggcccgg ggtgccccca aagcgggccc 121 gtgggggcct ctgggatgat gatgatgcac ctcggccatc ccaattcgag gaggacctgg 181 cactgatgga ggagatggag gcagaacaca ggctgcagga gcaggaggag gaggagctgc 241 agtcagtcct ggagggggtt gcagacgggc aggtcccacc atcagccata gatcctcgct 301 ggcttcggcc cacaccacca gcgctggacc cccagacaga gcccctcatc ttccaacagt 361 tggagattga ccattatgtg ggcccagcac agcctgtgcc tggggggccc ccaccatccc 421 acggctccgt gcctgtgctc cgcgccttcg gggtcaccga tgaggggttc tctgtctgct 481 gccacatcca cggcttcgct ccctacttct acaccccagc gccccctggt ttcgggcccg 541 agcacatggg tgacctgcaa cgggagctga acttggccat cagccgggac agtcgcgggg 601 ggagggagct gactgggccg gccgtgctgg ctgtggaact gtgctcccga gagagcatgt 661 ttgggtacca cgggcacggc ccctccccgt tcctgcgcat caccgtggcg ctgccgcgcc 721 tcgtggcccc ggcccgccgt ctcctggaac agggcatccg tgtggcaggc ctgggcacgc 781 ccagcttcgc gccctacgag gccaacgtcg actttgagat ccggttcatg gtggacacgg 841 acatcgtcgg ctgcaactgg ctggagctcc cagctgggaa atacgccctg aggctgaagg 901 agaaggctac gcagtgccag ctggaggcgg acgtgctgtg gtctgacgtg gtcagtcacc 961 caccggaagg gccatggcag cgcattgcgc ccttgcgcgt gctcagcttc gatatcgagt 1021 gcgccggccg caaaggcatc ttccctgagc ctgagcggga ccctgtcatc cagatctgct 1081 cgctgggcct gcgctggggg gagccggagc ccttcctacg cctggcgctc accctgcggc 1141 cctgtgcccc catcctgggt gccaaggtgc agagctacga gaaggaggag gacctgctgc 1201 aggcctggtc caccttcatc cgtatcatgg accccgacgt gatcaccggt tacaacatcc 1261 agaacttcga ccttccgtac ctcatctctc gggcccagac cctcaaggta caaacattcc 1321 ctttcctggg ccgtgtggcc ggcctttgct ccaacatccg ggactcttca ttccagtcca 1381 agcagacggg ccggcgggac accaaggttg tcagcatggt gggccgcgtg cagatggaca 1441 tgctgcaggt gctgctgcgg gagtacaagc tccgctccta cacgctcaat gccgtgagct 1501 tccacttcct gggcgagcag aaggaggacg tgcagcacag catcatcacc gacctgcaga 1561 atgggaacga ccagacccgc cgccgcctgg ctgtgtactg cctgaaggat gcctacctgc 1621 cactgcggct gctggagcgg ctcatggtgc tggtgaacgc cgtggagatg gcgagggtca 1681 ctggcgtgcc cctcagctac ctgctcagtc gtggccagca ggtcaaggtc gtatcccagc 1741 tgttgcggca ggccatgcac gaggggctgc tgatgcccgt ggtgaagtca gagggcggcg 1801 aggactacac gggagccact gtcatcgagc ccctcaaagg gtactacgac gtccccatcg 1861 ccaccctgga cttctcctcg ctgtacccgt ccatcatgat ggcccacaac ctgtgttaca 1921 ccacgctcct tcggcccggg actgcacaga aactgggcct gactgaggat cagttcatca 1981 ggacccccac cggggacgag tttgtgaaga cctcagtgcg gaaggggctg ctgccccaga 2041 tcctggagaa cctgctcagt gcccggaaga gggccaaggc cgagctggcc aaggagacag 2101 accccctccg gcgccaggtc ctggatggac ggcagctggc gctgaaggtg agcgccaact 2161 ccgtatacgg cttcactggc gcccaggtgg gcaagttgcc gtgcctggag atctcacaga 2221 gcgtcacggg gttcggacgt cagatgatcg agaaaaccaa gcagctggtg gagtctaagt 2281 acacagtgga gaatggctac agcaccagtg ccaaggtggt gtatggtgac actgactccg 2341 tcatgtgccg attcggcgtg tcctcggtgg ctgaggcgat ggccctgggg cgggaggccg 2401 cggactgggt gtcaggtcac ttcccgtcgc ccatccggct ggagtttgag aaggtctact 2461 tcccatacct gcttatcagc aagaagcgct acgcgggcct gctcttctcc tcccggcccg 2521 acgcccacga ccgcatggac tgcaagggcc tggaggccgt gcgcagggac aactgccccc 2581 tcgtggccaa cctggtcact gcctcactgc gccgcctgct catcgaccga gaccctgagg 2641 gcgcggtggc tcacgcacag gacgtcatct cggacctgct gtgcaaccgc atcgatatct 2701 cccagctggt catcaccaag gagctgaccc gcgcggcctc cgactatgcc ggcaagcagg 2761 cccacgtgga gctggccgag aggatgagga agcgggaccc cgggagtgcg cccagcctgg 2821 gcgaccgcgt cccctacgtg atcatcagtg ccgccaaggg tgtggccgcc tacatgaagt 2881 cggaggaccc gctgttcgtg ctggagcaca gcctgcccat tgacacgcag tactacctgg 2941 agcagcagct ggccaagccc ctcctgcgca tcttcgagcc catcctgggc gagggccgtg 3001 ccgaggctgt gctactgcgg ggggaccaca cgcgctgcaa gacggtgctc acgggcaagg 3061 tgggcggcct cctggccttc gccaaacgcc gcaactgctg cattggctgc cgcacagtgc 3121 TCAGCCACCA GGGAGCCGTG TGTGAGTTCT GCCAGCCCCG GGAGTCTGAG CTGTATCAGA 3181 AGGAGGTATC CCATCTGAAT GCCCTGGAGG AGCGCTTCTC GCGCCTCTGG ACGCAGTGCC 3241 AGCGCTGCCA GGGCAGCCTG CACGAGGACG TCATCTGCAC CAGCCGGGAC TGCCCCATCT 3301 TCTACATGCG CAAGAAGGTG CGGAAGGACC TGGAAGACCA GGAGCAGCTC CTGCGGCGCT 3361 TCGGACCCCC TGGACCTGAG GCCTGGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 3421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCCCCTAT 3541 TCGAATCAAA CGGGCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3601 aagatt