Construct: ORF TRCN0000478617
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014900.1_s317c1
- Derived from:
- ccsbBroadEn_07858
- DNA Barcode:
- ATAATACTTTAATGACCGAAGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLCL2 (23228)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478617
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23228 | PLCL2 | phospholipase C like 2 | NM_015184.5 | 99.8% | 99.5% | (many diffs) |
| 2 | human | 23228 | PLCL2 | phospholipase C like 2 | XM_017006025.1 | 99.8% | 99.5% | (many diffs) |
| 3 | human | 23228 | PLCL2 | phospholipase C like 2 | XM_006713073.3 | 97.7% | 97.4% | (many diffs) |
| 4 | human | 23228 | PLCL2 | phospholipase C like 2 | NM_001144382.2 | 88.6% | 88.3% | (many diffs) |
| 5 | human | 23228 | PLCL2 | phospholipase C like 2 | XM_017006022.2 | 88.6% | 88.3% | (many diffs) |
| 6 | human | 23228 | PLCL2 | phospholipase C like 2 | XM_017006023.1 | 88.6% | 88.3% | (many diffs) |
| 7 | human | 23228 | PLCL2 | phospholipase C like 2 | XM_017006024.2 | 88.6% | 88.3% | (many diffs) |
| 8 | mouse | 224860 | Plcl2 | phospholipase C-like 2 | NM_013880.3 | 79.1% | 86.3% | (many diffs) |
| 9 | mouse | 224860 | Plcl2 | phospholipase C-like 2 | XM_006524150.2 | 79.1% | 86.3% | (many diffs) |
| 10 | mouse | 224860 | Plcl2 | phospholipase C-like 2 | XM_006524151.3 | 79.1% | 86.3% | (many diffs) |
| 11 | mouse | 224860 | Plcl2 | phospholipase C-like 2 | XM_006524152.2 | 79.1% | 86.3% | (many diffs) |
| 12 | mouse | 224860 | Plcl2 | phospholipase C-like 2 | XM_006524153.3 | 72.3% | 78.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3069
- ORF length:
- 3003
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc aacagagaag aagatcagca gtgcaagtga ttgtattaat tcaatggttg 121 agggttcaga actcaaaaag gttcgctcca actctagaat ttatcatagg tactttttac 181 tggatgctga catgcagagc ctaaggtggg agccatctaa gaaggattct gagaaagcca 241 agattgacat taaatccatc aaggaagtga gaacaggaaa aaacacagac atattccgca 301 gcaatggcat ttctgaccgg atatctgaag attgtgcgtt ttccgtcata tatggagaga 361 attatgagtc actggatttg gttgccaact ccgcagatgt tgcaaacatc tgggttacag 421 gactgcggta cctaatttct tatggaaaac atacacttga tatgttagaa agtagccaag 481 ataacatgag gacttcttgg gtttcacaaa tgtttagtga aattgatgta gataaccttg 541 gacatataac tctgtgtaat gctgtgcaat gtatcagaaa cctcaatcct ggtttaaaaa 601 cgagcaaaat tgagcttaag ttcaaagaat tgcataaatc aaaggacaaa gctggtaccg 661 aggtcacaaa ggaagaattt attgaggttt ttcatgagct ttgtactaga cctgaaattt 721 atttcctttt agttcagttt tcaagcaata aagaattcct tgataccaag gaccttatga 781 tgtttcttga ggcagaacag ggtgtggcac atataaatga ggaaataagc cttgaaatta 841 ttcacaaata tgaaccatcc aaagagggtc aggaaaaggg ctggctctcc atagacgggt 901 tcactaatta ccttatgtca cctgactgtt atatattcga tccagaacat aagaaggtct 961 gtcaggatat gaagcaacct ctgtctcatt actttataaa ctcatctcat aatacatact 1021 taatagagga tcagttccga ggtccctccg acatcacagg atatattcga gctcttaaaa 1081 tgggttgccg gagtgttgaa ttagatgtat gggatgggcc ggacaatgaa cctgtaattt 1141 acacaggcca caccatgacc tctcagatag ttttccgcag tgtcattgat attattaaca 1201 agtatgcatt ctttgcttca gagtatcctc ttatcttgtg tttagaaaac cactgttcca 1261 ttaaacaaca gaaggtaatg gttcagcaca tgaagaaact tttaggagac aagctctata 1321 caacatcacc caatgttgag gaatcttatc taccatcccc agatgtcctg aaagggaaaa 1381 tactaattaa agcaaagaag ctgtcctcaa attgctctgg ggtagaagga gatgttactg 1441 acgaagatga aggagcagaa atgtctcaga ggatgggaaa agagaacatg gagcaaccca 1501 ataatgtgcc tgtgaagcga tttcagcttt gtaaagaact gtctgaactg gtcagcatct 1561 gcaaatcagt tcagttcaaa gaatttcaga tgtcgtttca ggttcagaag tactgggaag 1621 tctgttcctt taatgaagtg cttgccagca agtacgccaa tgaaaatcca ggggactttg 1681 taaattacaa caaacgtttt cttgctaggg tttttcccag tccaatgaga attgattcca 1741 gtaacatgaa tcctcaagat ttttggaaat gtggttgcca aattgtagcc atgaactttc 1801 agacaccagg actgatgatg gacctgaata ttggctggtt taggcagaac ggaaactgtg 1861 gctatgtcct ccggccagcc atcatgaggg aggaggtctc cttcttcagc gtcaatacaa 1921 aagactctgt cccaggggtc tcacctcaac ttcttcacat taaaatcatc agtgggcaga 1981 actttcccaa gcccaaagga tcaggtgcca aaggtgatgt ggtagatcct tatgtctatg 2041 ttgaaatcca tggaatccct gctgattgtg cagaacaaag gacaaaaaca gtgcaccaga 2101 atggagacgc tcacattttt gatgaaagct ttgaatttca aatcaacctg cctgaactgg 2161 ccatggtgcg ctttgtagtg ctggatgatg actacattgg ggatgaattc atcggccagt 2221 acacaattcc ctttgaatgt ttacagacgg gctaccgcca tgtccccctg cagtccttaa 2281 ctggagaggt ccttgcacat gcttctttat ttgtccacgt ggctattact aaccgaagag 2341 gaggaggaaa gcctcgtaaa aggggccttt ctgtgagaaa agggaagaaa tccagggaat 2401 atgcatcttt gagaacactg tggattaaaa ccgtggatga ggtattcaag aatgcccagc 2461 cccctatacg ggatgccaca gatctgagag aaaacatgca gaatgcggtg gtgtcattca 2521 aggagctgtg tggcctctcc tctgtggcca atctcatgca gtgcatgttg gcggtgtctc 2581 cccgctttct ggggcccgat aacacacccc tagtggtcct aaatctcagc gagcagtacc 2641 ccacaatgga gctgcaggga attgtgccgg aggttctgaa gaagatcgta acaacttatg 2701 acatgatgat tcagtccctc aaggcgttga ttGAAAATGC AGATGCTGTA TATGAAAAGA 2761 TCGTACATTG TCAGAAGGCA GCCATGGAAT TCCATGAACA CTTGCACAGC ATAGGCACCA 2821 AGGAAGGTTT GAAGGAAAGA AAACTACAAA AAGCAGTGGA GAGCTTTACC TGGAATATTA 2881 CCATCTTAAA GGGACAAGCA GATCTTTTGA AATATGCTAA GAATGAGACA TTGGAGAACC 2941 TGAAACAAAT CCATTTTGCT GCTGTTTCAT GTGGACTGAA TAAACCAGGC ACCGAAAATG 3001 CTGATGTCCA GAAGCCACGC CGGAGCTTGG AAGTCATACC CGAAAAAGCA AACGATGAAA 3061 CTGGAGAATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3121 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGCCC GTAACTTGAA AGTATTTCGA 3181 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATAATA CTTTAATGAC CGAAGTTCAC 3241 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt