Construct: ORF TRCN0000478617

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014900.1_s317c1
Derived from:
ccsbBroadEn_07858
DNA Barcode:
ATAATACTTTAATGACCGAAGTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PLCL2 (23228)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478617
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23228 PLCL2 phospholipase C like 2 NM_015184.5 99.8% 99.5% (many diffs)
2 human 23228 PLCL2 phospholipase C like 2 XM_017006025.1 99.8% 99.5% (many diffs)
3 human 23228 PLCL2 phospholipase C like 2 XM_006713073.3 97.7% 97.4% (many diffs)
4 human 23228 PLCL2 phospholipase C like 2 NM_001144382.2 88.6% 88.3% (many diffs)
5 human 23228 PLCL2 phospholipase C like 2 XM_017006022.2 88.6% 88.3% (many diffs)
6 human 23228 PLCL2 phospholipase C like 2 XM_017006023.1 88.6% 88.3% (many diffs)
7 human 23228 PLCL2 phospholipase C like 2 XM_017006024.2 88.6% 88.3% (many diffs)
8 mouse 224860 Plcl2 phospholipase C-like 2 NM_013880.3 79.1% 86.3% (many diffs)
9 mouse 224860 Plcl2 phospholipase C-like 2 XM_006524150.2 79.1% 86.3% (many diffs)
10 mouse 224860 Plcl2 phospholipase C-like 2 XM_006524151.3 79.1% 86.3% (many diffs)
11 mouse 224860 Plcl2 phospholipase C-like 2 XM_006524152.2 79.1% 86.3% (many diffs)
12 mouse 224860 Plcl2 phospholipase C-like 2 XM_006524153.3 72.3% 78.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3069
ORF length:
3003
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc aacagagaag aagatcagca gtgcaagtga ttgtattaat tcaatggttg
121agggttcaga actcaaaaag gttcgctcca actctagaat ttatcatagg tactttttac
181tggatgctga catgcagagc ctaaggtggg agccatctaa gaaggattct gagaaagcca
241agattgacat taaatccatc aaggaagtga gaacaggaaa aaacacagac atattccgca
301gcaatggcat ttctgaccgg atatctgaag attgtgcgtt ttccgtcata tatggagaga
361attatgagtc actggatttg gttgccaact ccgcagatgt tgcaaacatc tgggttacag
421gactgcggta cctaatttct tatggaaaac atacacttga tatgttagaa agtagccaag
481ataacatgag gacttcttgg gtttcacaaa tgtttagtga aattgatgta gataaccttg
541gacatataac tctgtgtaat gctgtgcaat gtatcagaaa cctcaatcct ggtttaaaaa
601cgagcaaaat tgagcttaag ttcaaagaat tgcataaatc aaaggacaaa gctggtaccg
661aggtcacaaa ggaagaattt attgaggttt ttcatgagct ttgtactaga cctgaaattt
721atttcctttt agttcagttt tcaagcaata aagaattcct tgataccaag gaccttatga
781tgtttcttga ggcagaacag ggtgtggcac atataaatga ggaaataagc cttgaaatta
841ttcacaaata tgaaccatcc aaagagggtc aggaaaaggg ctggctctcc atagacgggt
901tcactaatta ccttatgtca cctgactgtt atatattcga tccagaacat aagaaggtct
961gtcaggatat gaagcaacct ctgtctcatt actttataaa ctcatctcat aatacatact
1021taatagagga tcagttccga ggtccctccg acatcacagg atatattcga gctcttaaaa
1081tgggttgccg gagtgttgaa ttagatgtat gggatgggcc ggacaatgaa cctgtaattt
1141acacaggcca caccatgacc tctcagatag ttttccgcag tgtcattgat attattaaca
1201agtatgcatt ctttgcttca gagtatcctc ttatcttgtg tttagaaaac cactgttcca
1261ttaaacaaca gaaggtaatg gttcagcaca tgaagaaact tttaggagac aagctctata
1321caacatcacc caatgttgag gaatcttatc taccatcccc agatgtcctg aaagggaaaa
1381tactaattaa agcaaagaag ctgtcctcaa attgctctgg ggtagaagga gatgttactg
1441acgaagatga aggagcagaa atgtctcaga ggatgggaaa agagaacatg gagcaaccca
1501ataatgtgcc tgtgaagcga tttcagcttt gtaaagaact gtctgaactg gtcagcatct
1561gcaaatcagt tcagttcaaa gaatttcaga tgtcgtttca ggttcagaag tactgggaag
1621tctgttcctt taatgaagtg cttgccagca agtacgccaa tgaaaatcca ggggactttg
1681taaattacaa caaacgtttt cttgctaggg tttttcccag tccaatgaga attgattcca
1741gtaacatgaa tcctcaagat ttttggaaat gtggttgcca aattgtagcc atgaactttc
1801agacaccagg actgatgatg gacctgaata ttggctggtt taggcagaac ggaaactgtg
1861gctatgtcct ccggccagcc atcatgaggg aggaggtctc cttcttcagc gtcaatacaa
1921aagactctgt cccaggggtc tcacctcaac ttcttcacat taaaatcatc agtgggcaga
1981actttcccaa gcccaaagga tcaggtgcca aaggtgatgt ggtagatcct tatgtctatg
2041ttgaaatcca tggaatccct gctgattgtg cagaacaaag gacaaaaaca gtgcaccaga
2101atggagacgc tcacattttt gatgaaagct ttgaatttca aatcaacctg cctgaactgg
2161ccatggtgcg ctttgtagtg ctggatgatg actacattgg ggatgaattc atcggccagt
2221acacaattcc ctttgaatgt ttacagacgg gctaccgcca tgtccccctg cagtccttaa
2281ctggagaggt ccttgcacat gcttctttat ttgtccacgt ggctattact aaccgaagag
2341gaggaggaaa gcctcgtaaa aggggccttt ctgtgagaaa agggaagaaa tccagggaat
2401atgcatcttt gagaacactg tggattaaaa ccgtggatga ggtattcaag aatgcccagc
2461cccctatacg ggatgccaca gatctgagag aaaacatgca gaatgcggtg gtgtcattca
2521aggagctgtg tggcctctcc tctgtggcca atctcatgca gtgcatgttg gcggtgtctc
2581cccgctttct ggggcccgat aacacacccc tagtggtcct aaatctcagc gagcagtacc
2641ccacaatgga gctgcaggga attgtgccgg aggttctgaa gaagatcgta acaacttatg
2701acatgatgat tcagtccctc aaggcgttga ttGAAAATGC AGATGCTGTA TATGAAAAGA
2761TCGTACATTG TCAGAAGGCA GCCATGGAAT TCCATGAACA CTTGCACAGC ATAGGCACCA
2821AGGAAGGTTT GAAGGAAAGA AAACTACAAA AAGCAGTGGA GAGCTTTACC TGGAATATTA
2881CCATCTTAAA GGGACAAGCA GATCTTTTGA AATATGCTAA GAATGAGACA TTGGAGAACC
2941TGAAACAAAT CCATTTTGCT GCTGTTTCAT GTGGACTGAA TAAACCAGGC ACCGAAAATG
3001CTGATGTCCA GAAGCCACGC CGGAGCTTGG AAGTCATACC CGAAAAAGCA AACGATGAAA
3061CTGGAGAATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
3121CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGCCC GTAACTTGAA AGTATTTCGA
3181TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATAATA CTTTAATGAC CGAAGTTCAC
3241GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)