Construct: ORF TRCN0000478644
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015821.1_s317c1
- Derived from:
- ccsbBroadEn_05122
- DNA Barcode:
- CACTAGAAGGCTCACCATTACCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEDD2 (162989)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478644
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 162989 | DEDD2 | death effector domain conta... | NM_001270614.2 | 99.7% | 100% | 738T>C;807C>T |
| 2 | human | 162989 | DEDD2 | death effector domain conta... | NM_133328.4 | 99.7% | 100% | 738T>C;807C>T |
| 3 | human | 162989 | DEDD2 | death effector domain conta... | NM_001270615.2 | 98.2% | 98.4% | 432_433insGGTCAGTGGGAGACA;723T>C;792C>T |
| 4 | human | 162989 | DEDD2 | death effector domain conta... | XM_011526569.1 | 88.8% | 89% | 589_708del;858T>C;927C>T |
| 5 | human | 162989 | DEDD2 | death effector domain conta... | XM_017026402.1 | 88.8% | 89% | 589_708del;858T>C;927C>T |
| 6 | human | 162989 | DEDD2 | death effector domain conta... | XM_024451400.1 | 88.8% | 89% | 589_708del;858T>C;927C>T |
| 7 | human | 162989 | DEDD2 | death effector domain conta... | XM_011526572.1 | 74.5% | 61.5% | 589_610del;760T>C;768_769ins232 |
| 8 | human | 162989 | DEDD2 | death effector domain conta... | XM_011526571.1 | 74.1% | 56.2% | 588_685del;836T>C;897_898ins179 |
| 9 | human | 162989 | DEDD2 | death effector domain conta... | XM_017026403.1 | 68.7% | 55.6% | 589_770del;920T>C;981_982ins179 |
| 10 | human | 162989 | DEDD2 | death effector domain conta... | XR_935758.2 | 56.9% | 1_123del;712_795del;882_883ins303 | |
| 11 | human | 162989 | DEDD2 | death effector domain conta... | NR_073047.2 | 37.9% | (many diffs) | |
| 12 | human | 162989 | DEDD2 | death effector domain conta... | NR_073048.2 | 37.3% | (many diffs) | |
| 13 | human | 162989 | DEDD2 | death effector domain conta... | NR_073049.2 | 36.9% | (many diffs) | |
| 14 | human | 162989 | DEDD2 | death effector domain conta... | NR_073050.2 | 36.5% | (many diffs) | |
| 15 | human | 162989 | DEDD2 | death effector domain conta... | NR_073051.2 | 35.7% | (many diffs) | |
| 16 | human | 162989 | DEDD2 | death effector domain conta... | NR_073046.2 | 35.4% | (many diffs) | |
| 17 | human | 162989 | DEDD2 | death effector domain conta... | NR_073052.2 | 29.6% | (many diffs) | |
| 18 | mouse | 67379 | Dedd2 | death effector domain-conta... | NM_207677.3 | 87.4% | 92.1% | (many diffs) |
| 19 | mouse | 67379 | Dedd2 | death effector domain-conta... | XM_006540322.1 | 87.4% | 92.1% | (many diffs) |
| 20 | mouse | 67379 | Dedd2 | death effector domain-conta... | XR_391094.2 | 60.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctatccggg tcgaccccgg ccccgtgctg ggaggaggat gagtgcctgg 121 actactacgg gatgctgtcg cttcaccgta tgttcgaggt ggtgggcggg caactgaccg 181 agtgcgagct ggagctcctg gcctttctgc tggatgaggc tcctggcgcc gccggaggct 241 tagcccgggc ccgcagcggc ctagagctcc tgctggagct ggagcgccgc gggcagtgcg 301 acgagagcaa cctgcggctg ctggggcaac tcctgcgcgt gctggcccgc cacgacctgc 361 tgccgcacct ggcgcgcaag cggcgccggc cagtgtctcc agaacgctat agctatggca 421 cctccagctc ttcaaagagg acagagggta gctgccgtcg ccgtcggcag tcaagcagtt 481 ctgcaaattc tcagcagggt cagtgggaga caggctcccc cccaaccaag cggcagcggc 541 ggagtcgggg ccggcccagt ggtggtgcca gacggcggcg gagaggggcc ccagccgcac 601 cccagcagca gtcagagccc gccagacctt cctctgaagg caaagtgacc tgtgacatcc 661 ggctccgggt tcgagcagag tactgcgagc atgggccagc cttggagcag ggcgtggcat 721 cccggcggcc ccaggcgctg gcgcggcagc tggacgtgtt tgggcaggcc accgcagtgc 781 tgcgctcaag ggacctgggc tccgtggttt gtgacatcaa gttctcagag ctctcctATC 841 TGGACGCCTT CTGGGGCGAC TACCTGAGTG GTGCCCTGCT GCAGGCCCTG CGGGGCGTGT 901 TCCTGACTGA GGCCCTGCGA GAGGCTGTGG GCCGGGAGGC TGTTCGCCTG CTGGTCAGTG 961 TGGATGAGGC TGACTATGAG GCTGGCCGGC GCCGCCTGTT GCTGATGGAG GAGGAAGGGG 1021 GGCGGCGCCC GACAGAGGCC TCCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC ACTAGAAGGC 1201 TCACCATTAC CCTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att