Construct: ORF TRCN0000478645
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014555.1_s317c1
- Derived from:
- ccsbBroadEn_12089
- DNA Barcode:
- GCGCTTACTCCTGTGCGCTGACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZCCHC10 (54819)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478645
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001300816.3 | 100% | 100% | |
| 2 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001300817.3 | 96.8% | 96.8% | 309_310insAGGTCTAAGAGTGTAACC |
| 3 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001308127.2 | 95% | 95% | 106_135del |
| 4 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001300818.3 | 92.7% | 92.7% | 265_266ins42 |
| 5 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_017665.4 | 88.5% | 88.5% | 41_42ins66 |
| 6 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | XM_011543502.1 | 88.1% | 88.1% | 106_135del;295_296ins42 |
| 7 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001300819.3 | 81.2% | 81.2% | 41_42ins66;199_200ins42 |
| 8 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001308128.2 | 60.4% | 60.4% | 41_42ins228 |
| 9 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001308129.2 | 53.1% | 53.1% | 41_42ins270 |
| 10 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001300822.3 | 51.9% | 47.9% | 268_295del;342_343ins262 |
| 11 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | XM_011543503.2 | 49.5% | 45.6% | 106_135del;298_325del;372_373ins262 |
| 12 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001308130.2 | 41% | 36.8% | 41_42ins66;202_229del;276_277ins262 |
| 13 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NR_131766.2 | 24.4% | (many diffs) | |
| 14 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NR_125337.3 | 23.3% | (many diffs) | |
| 15 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NR_131767.2 | 23% | (many diffs) | |
| 16 | mouse | 67966 | Zcchc10 | zinc finger, CCHC domain co... | NM_026479.4 | 70.6% | 74.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gactcccatg catcggctaa tagcccggag acaagcattc gacacagagt 121 tgcagcctgt caagaccttt tggatcctga ttcagccatc catcgttatt agtgaagcaa 181 ataagcaaca tgtaagatgt cagaaatgct tggaatttgg acattggact tatgaatgca 241 caggaaaaag aaaataccta cataggcccT CAAGGACAGC AGAACTAAAG AAAGCTTTAA 301 AAGAAAAAGA AAACAGATTA TTATTGCAAC AAAGCATTGG AGAAACCAAT GTAGAAAGAA 361 AGGCCAAGAA AAAAAGGTCT AAGAGTGTAA CCAGTTCCAG TAGCAGTAGC AGTGACAGTT 421 CTGCCAGTGA TTCTTCATCA GAGAGTGAAG AAACATCTAC CTCTTCCTCC TCAGAGGACA 481 GTGACACTGA TGAAAGCTCC TCTAGTTCCT CATCCTCAGC CTCCTCCACA ACCTCTTCCT 541 CCTCCTCTGA TTCAGACTCA GATTCCAGCT CTTCCAGTAG CAGTAGCACC AGCACAGATA 601 GCAGCTCTGA CGATGAACCA CCAAAGAAGA AGAAAAAGAA ATACCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGAGCG CTTACTCCTG TGCGCTGACC TACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t