Construct: ORF TRCN0000478659
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002701.1_s317c1
- Derived from:
- ccsbBroadEn_01800
- DNA Barcode:
- CAACTTGGCCTGCCTCGGGAAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZSCAN21 (7589)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478659
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7589 | ZSCAN21 | zinc finger and SCAN domain... | NM_001362779.1 | 100% | 100% | |
| 2 | human | 7589 | ZSCAN21 | zinc finger and SCAN domain... | NM_001362780.1 | 100% | 100% | |
| 3 | human | 7589 | ZSCAN21 | zinc finger and SCAN domain... | NM_145914.3 | 100% | 100% | |
| 4 | human | 7589 | ZSCAN21 | zinc finger and SCAN domain... | XM_005250568.4 | 100% | 100% | |
| 5 | human | 7589 | ZSCAN21 | zinc finger and SCAN domain... | XM_006716113.3 | 100% | 100% | |
| 6 | human | 7589 | ZSCAN21 | zinc finger and SCAN domain... | NM_001362781.2 | 90.5% | 55.2% | 782_783ins103;1317_1350del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1485
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gaccaaggta ctaggcatgg ccccagttct gggccctagg cctccacagg 121 agcaggtggg gcctctgatg gtaaaagtcg aggagaaaga agagaaaggc aagtaccttc 181 ctagcctgga gatgttccgc cagcgcttca ggcagtttgg gtaccatgat acccctggac 241 cccgagaggc cctgagccaa ctccgggtgc tctgctgtga gtggctgagg cccgagatcc 301 acaccaagga gcagatcctg gagctactgg tgctggagca gttcctgacc atcctgcccc 361 aggagctcca ggcctgggtg caggagcatt gcccggagag cgctgaagag gctgtcactc 421 tcctcgaaga tctggagcgg gaactggatg agccaggaca ccaggtctca actcctccaa 481 acgaacagaa accggtgtgg gagaagatat cctcctcagg aactgcaaag gaatccccga 541 gcagcatgca gccacagccc ttggagacca gtcacaaata cgagtcttgg gggcccctgt 601 acatccaaga gtctggtgag gagcaggagt tcgctcaaga tccaagaaag gtccgagatt 661 gcagattgag tacccagcac gaggaatcag cagatgagca gaaaggttct gaagcagagg 721 ggctcaaagg ggatataatt tctgtgatta tcgccaataa acctgaggcc agcttagaga 781 ggcagtgcgt aaaccttgaa aatgaaaaag gaacaaaacc ccctcttcaa gaggcaggct 841 ccaagaaagg tagagaatca gttcctacta aacctacccc aggagagaga cgttatatat 901 gtgctgaatg tggcaaagcc tttagtaata gctcaaatct caccaaacac aggagaacac 961 acactgggga gaaaccttac gtgtgcacca agtgtgggaa agctttcagc cacagctcaa 1021 acctcaccct ccactacaga acacacttgg tggaccggcc ctatgactgt aagtgtggaa 1081 aagcttttgg gcagagctca gaccTTCTTA AACATCAGAG AATGCACACA GAAGAGGCGC 1141 CATATCAGTG CAAAGATTGT GGCAAGGCTT TCAGCGGGAA AGGCAGCCTC ATTCGTCACT 1201 ATCGGATCCA CACTGGGGAG AAGCCTTATC AGTGTAACGA ATGTGGGAAG AGCTTCAGTC 1261 AGCATGCGGG CCTCAGCTCC CACCAGAGAC TCCACACCGG AGAGAAGCCA TATAAGTGTA 1321 AGGAGTGTGG GAAAGCCTTC AACCACAGCT CCAACTTCAA TAAACACCAC AGAATCCACA 1381 CCGGGGAAAA GCCCTACTGG TGTCATCACT GTGGAAAGAC CTTCTGTAGC AAGTCCAATC 1441 TTTCCAAACA TCAGCGAGTC CACACTGGAG AGGGAGAAGC ACCGTACCCA ACTTTCTTGT 1501 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1561 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1621 GAAAGGACGA CAACTTGGCC TGCCTCGGGA AATTACGCGT TAAGTCgaca atcaacctct 1681 ggattacaaa atttgtgaaa gatt