Construct: ORF TRCN0000478677

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014940.1_s317c1
Derived from:
ccsbBroadEn_11250
DNA Barcode:
TTAGCGGAAAGTACGATTCTGTGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSCA (8000)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478677
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8000 PSCA prostate stem cell antigen NM_005672.5 92.1% 92.6% 0_1ins27;285T>C;342C>A
2 human 8000 PSCA prostate stem cell antigen NR_033343.2 29.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
90
ORF end:
459
ORF length:
369
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAAGGCTGT GCTGCTTGCC CTGTTGATGG
121CAGGCTTGGC CCTGCAGCCA GGCACTGCCC TGCTGTGCTA CTCCTGCAAA GCCCAGGTGA
181GCAACGAGGA CTGCCTGCAG GTGGAGAACT GCACCCAGCT GGGGGAGCAG TGCTGGACCG
241CGCGCATCCG CGCAGTTGGC CTCCTGACCG TCATCAGCAA AGGCTGCAGC TTGAACTGCG
301TGGATGACTC ACAGGACTAC TACGTGGGCA AGAAGAACAT CACGTGCTGT GACACCGACT
361TGTGCAACGC CAGCGGGGCC CATGCCCTGC AGCCGGCTGC CGCCATCCTT GCGCTGCTCC
421CTGCACTCGG CCTGCTGCTC TGGGGACCCG GCCAGCTATA CCCAACTTTC TTGTACAAAG
481TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
541GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
601ACGATTAGCG GAAAGTACGA TTCTGTGTAC GCGTTAAGTC gacaatcaac ctctggatta
661caaaatttgt gaaagatt

Download FASTA (ORF) (Full)