Construct: ORF TRCN0000478677
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014940.1_s317c1
- Derived from:
- ccsbBroadEn_11250
- DNA Barcode:
- TTAGCGGAAAGTACGATTCTGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSCA (8000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478677
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8000 | PSCA | prostate stem cell antigen | NM_005672.5 | 92.1% | 92.6% | 0_1ins27;285T>C;342C>A |
| 2 | human | 8000 | PSCA | prostate stem cell antigen | NR_033343.2 | 29.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 459
- ORF length:
- 369
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAAGGCTGT GCTGCTTGCC CTGTTGATGG 121 CAGGCTTGGC CCTGCAGCCA GGCACTGCCC TGCTGTGCTA CTCCTGCAAA GCCCAGGTGA 181 GCAACGAGGA CTGCCTGCAG GTGGAGAACT GCACCCAGCT GGGGGAGCAG TGCTGGACCG 241 CGCGCATCCG CGCAGTTGGC CTCCTGACCG TCATCAGCAA AGGCTGCAGC TTGAACTGCG 301 TGGATGACTC ACAGGACTAC TACGTGGGCA AGAAGAACAT CACGTGCTGT GACACCGACT 361 TGTGCAACGC CAGCGGGGCC CATGCCCTGC AGCCGGCTGC CGCCATCCTT GCGCTGCTCC 421 CTGCACTCGG CCTGCTGCTC TGGGGACCCG GCCAGCTATA CCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGATTAGCG GAAAGTACGA TTCTGTGTAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt