Construct: ORF TRCN0000478689
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010757.2_s317c1
- Derived from:
- ccsbBroadEn_07839
- DNA Barcode:
- GTTCCGTTCACCGACTGAGGGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPART (23111)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478689
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23111 | SPART | spartin | NM_001142294.1 | 99.8% | 99.6% | 205C>A;1250C>A;1629A>G |
| 2 | human | 23111 | SPART | spartin | NM_001142295.1 | 99.8% | 99.6% | 205C>A;1250C>A;1629A>G |
| 3 | human | 23111 | SPART | spartin | NM_001142296.2 | 99.8% | 99.6% | 205C>A;1250C>A;1629A>G |
| 4 | human | 23111 | SPART | spartin | NM_015087.5 | 99.8% | 99.6% | 205C>A;1250C>A;1629A>G |
| 5 | human | 23111 | SPART | spartin | XM_005266313.5 | 99.8% | 99.6% | 205C>A;1250C>A;1629A>G |
| 6 | human | 23111 | SPART | spartin | XM_005266314.3 | 99.8% | 99.6% | 205C>A;1250C>A;1629A>G |
| 7 | human | 23111 | SPART | spartin | XM_005266315.3 | 99.8% | 99.6% | 205C>A;1250C>A;1629A>G |
| 8 | human | 23111 | SPART | spartin | XM_005266317.3 | 99.8% | 99.6% | 205C>A;1250C>A;1629A>G |
| 9 | human | 23111 | SPART | spartin | XM_011535012.2 | 99.8% | 99.6% | 205C>A;1250C>A;1629A>G |
| 10 | human | 23111 | SPART | spartin | XM_024449334.1 | 99.8% | 99.6% | 205C>A;1250C>A;1629A>G |
| 11 | human | 23111 | SPART | spartin | XR_001749523.2 | 39.3% | (many diffs) | |
| 12 | mouse | 229285 | Spg20 | spastic paraplegia 20, spar... | NM_001144987.1 | 80.6% | 84.8% | (many diffs) |
| 13 | mouse | 229285 | Spg20 | spastic paraplegia 20, spar... | NM_144895.2 | 80.6% | 84.8% | (many diffs) |
| 14 | mouse | 229285 | Spg20 | spastic paraplegia 20, spar... | XM_006501320.2 | 80.6% | 84.8% | (many diffs) |
| 15 | mouse | 229285 | Spg20 | spastic paraplegia 20, spar... | NM_001144988.1 | 75.3% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2064
- ORF length:
- 1998
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcaagagcca caaaatggag aacctgctga aattaagatc atcagagaag 121 catataagaa ggccttttta tttgttaaca aaggtctgaa tacagatgaa ttaggtcaga 181 aggaagaagc aaagaactac tataagcaag gaataggaca cctgctcaga gggatcagca 241 tttcatcaaa agagtctgaa cacacaggta ctgggtggga atctgctaga cagatgcaac 301 agaaaatgaa agaaactcta cagaatgtac gcaccaggct ggaaattcta gagaagggtc 361 ttgccacttc tctgcagaat gatcttcagg aggtgcccaa gttatatcca gaatttccac 421 ctaaagacat gtgtgaaaaa ttaccagagc ctcagtcttt tagttcagct cctcagcatg 481 ctgaagtaaa tggaaacacc tcaactccaa gtgcaggggc agttgctgca cctgcttctc 541 tgtctttacc atcacaaagt tgtccagcag aagctcctcc tgcttatact cctcaagctg 601 ctgaaggtca ctacactgta tcctatggaa cagattctgg ggagttttca tcagttggag 661 aggagtttta taggaatcat tctcagccac cgcctcttga gaccttaggg ctggatgcag 721 atgaattgat tttgatacca aatggagtac agattttttt tgtaaatcct gcaggggagg 781 ttagtgcacc ttcgtatcct gggtaccttc gaattgtgag gtttttggat aattctctcg 841 atacggttct aaaccgtcct cccgggtttc ttcaggtttg tgactggtta tatcctctag 901 ttcctgatag atctccggtt ctgaaatgta ctgcgggagc ctacatgttt cctgatacaa 961 tgctacaagc agcaggatgc tttgtggggg tcgtcctgtc ctctgagtta ccagaggatg 1021 atagagagct ctttgaggat ctgttaaggc aaatgtctga ccttcggctc caggccaact 1081 ggaacagagc agaagaagaa aatgaattcc aaatccctgg aagaactaga ccctcctctg 1141 accaactaaa agaagcctct ggcactgatg tgaaacagtt ggaccaaggc aataaggatg 1201 tacgtcataa aggaaaacgt ggaaaaaggg ctaaagatac ttcaagtgaa gaagttaacc 1261 tgagtcacat tgtaccatgt gagccagttc cagaagaaaa gccaaaagaa ttacatgaat 1321 ggagtgaaaa agtggctcac aacattttgt caggtgcttc ctgggtgagt tggggtttag 1381 tcaaaggtgc tgagattact ggtaaggcaa tccagaaagg tgcttctaaa ctccgagagc 1441 ggattcaacc agaagaaaaa cccgtggaag ttagtccagc tgtcaccaag ggactttata 1501 tagcgaagca agctacagga ggagcagcaa aagtcagtca gttcctggtt gatggagttt 1561 gcactgtagc aaattgcgtt ggaaaagaac tagctccaca tgtcaagaag catggaagca 1621 aacttgttcc agaatctctt aaaaaagaca aagatgggaa atctcctctg gatggtgcta 1681 tggttgtagc agcgagtagt gttcaaggat tttcaactgt ctggcaagga ttggaatgtg 1741 cagctaaatg catcgttaac aatgtttcag cagaaactgt acaaactgtc agatacaaat 1801 acggatataa tgcaggagaa gctacccacc atgcggtgga ttctgcggtc aatgttggcg 1861 taactgccta caatattAAC AACATTGGTA TCAAAGCAAT GGTGAAGAAA ACTGCAACAC 1921 AAACAGGACA CACTCTCCTT GAGGACTATC AGATAGTTGA TAATTCTCAG AGGGAAAATC 1981 AAGAAGGAGC AGCAAATGTC AACGTGAGAG GGGAGAAGGA TGAGCAGACG AAGGAAGTAA 2041 AGGAGGCAAA GAAGAAAGAT AAATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2101 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2161 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG TTCCGTTCAC 2221 CGACTGAGGG TAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2281 att