Construct: ORF TRCN0000478717
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000948.1_s317c1
- Derived from:
- ccsbBroadEn_08885
- DNA Barcode:
- ACTGAGACACCTTGAGGCGTACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL11 (65003)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478717
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65003 | MRPL11 | mitochondrial ribosomal pro... | NM_016050.5 | 99.8% | 100% | 57G>A |
| 2 | human | 65003 | MRPL11 | mitochondrial ribosomal pro... | NM_170739.3 | 89.3% | 81.4% | (many diffs) |
| 3 | human | 65003 | MRPL11 | mitochondrial ribosomal pro... | XM_024448661.1 | 89.3% | 81.4% | (many diffs) |
| 4 | human | 65003 | MRPL11 | mitochondrial ribosomal pro... | NM_170738.4 | 86.4% | 86.4% | 43_44ins78 |
| 5 | human | 65003 | MRPL11 | mitochondrial ribosomal pro... | XM_017018135.2 | 76.1% | 68% | (many diffs) |
| 6 | human | 65003 | MRPL11 | mitochondrial ribosomal pro... | NR_120588.1 | 35.3% | (many diffs) | |
| 7 | human | 65003 | MRPL11 | mitochondrial ribosomal pro... | NR_120589.1 | 33% | (many diffs) | |
| 8 | mouse | 66419 | Mrpl11 | mitochondrial ribosomal pro... | NM_025553.4 | 85.2% | 87.5% | (many diffs) |
| 9 | mouse | 66419 | Mrpl11 | mitochondrial ribosomal pro... | XM_006531821.2 | 56.1% | 57.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aaagctcggc cgggccgccc ggggcctcag gaagcccgag gtcggcggtg 121 taatccgggc gatcgtgcgg gcaggcctgg ccatgcccgg gcccccacta ggcccagtgc 181 tgggtcagag aggcgtttcc atcaaccagt tttgcaagga gttcaatgag aggacaaagg 241 acatcaagga aggcattcct ctgcctacca agattttagt gaagcctgac aggacatttg 301 aaattaagat tggacagccc actgtttccT ACTTCCTGAA GGCAGCAGCT GGGATTGAAA 361 AGGGGGCCCG GCAAACAGGG AAAGAGGTGG CAGGCCTGGT GACCTTGAAG CATGTGTATG 421 AGATTGCCCG CATCAAAGCT CAGGATGAGG CATTTGCCCT GCAGGATGTA CCCCTGTCGT 481 CTGTTGTCCG CTCCATCATC GGGTCTGCCC GTTCTCTGGG CATTCGCGTG GTGAAGGACC 541 TCAGTTCAGA AGAGCTTGCA GCTTTCCAGA AGGAACGAGC CATCTTCCTG GCTGCTCAGA 601 AGGAGGCAGA TTTGGCTGCC CAAGAAGAAG CTGCCAAGAA GTGCCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGAACT GAGACACCTT GAGGCGTACA TACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t