Construct: ORF TRCN0000478720
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013733.1_s317c1
- Derived from:
- ccsbBroadEn_03849
- DNA Barcode:
- ACGTCCCGCTACGTATGTTGGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GBA3 (57733)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478720
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57733 | GBA3 | glucosylceramidase beta 3 (... | NM_001128432.2 | 100% | 100% | |
| 2 | human | 57733 | GBA3 | glucosylceramidase beta 3 (... | NR_102356.2 | 39.9% | 1_79del;526A>T;566_1214del | |
| 3 | human | 57733 | GBA3 | glucosylceramidase beta 3 (... | NM_020973.4 | 34.5% | 34.5% | 283_1203del |
| 4 | human | 57733 | GBA3 | glucosylceramidase beta 3 (... | NM_001277225.1 | 32.2% | 30.1% | (many diffs) |
| 5 | human | 57733 | GBA3 | glucosylceramidase beta 3 (... | NR_102355.2 | 22.7% | (many diffs) | |
| 6 | human | 57733 | GBA3 | glucosylceramidase beta 3 (... | NR_102357.2 | 21.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tttccctgca ggatttggat gggcggcagc cactgcagct tatcaagtag 121 aaggaggctg ggatgcagat ggaaaaggcc cttgtgtctg ggacacattt actcatcagg 181 gaggagagag agttttcaag aaccagactg gcgatgtagc ttgtggcagc tacactctgt 241 gggaggaaga ttTGAAATGT ATCAAACAGC TTGGATTGAC TCATTACCGC TTCTCTCTTT 301 CCTGGTCACG TCTGTTACCT GATGGGACGA CAGGTTTCAT CAACCAGAAA GCTATCCAAC 361 TTGATAAAGT CAATCTTCAA GTATATTGTG CATGGTCTCT TCTGGATAAC TTTGAGTGGA 421 ACCAGGGATA CAGCAGCCGG TTTGGTCTCT TCCACGTTGA TTTTGAAGAC CCAGCTAGAC 481 CCCGAGTCCC TTACACATCG GCCAAGGAAT ATGCCAAGAT CATCCGAAAC AATGGCCTTG 541 AAGCACATCT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACG TCCCGCTACG TATGTTGGGC 721 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t