Transcript: Human NR_102356.2

Homo sapiens glucosylceramidase beta 3 (gene/pseudogene) (GBA3), transcript variant 2, non-coding, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
GBA3 (57733)
Length:
1214
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_102356.2
NBCI Gene record:
GBA3 (57733)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_102356.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416767 ATCAATGGATCTGTGATTAAA pLKO_005 677 3UTR 100% 15.000 10.500 N GBA3 n/a
2 TRCN0000433946 TAATGCCTCGTGAAGTATTTA pLKO_005 710 3UTR 100% 15.000 10.500 N GBA3 n/a
3 TRCN0000049591 GACAGGTTTCATCAACCAGAA pLKO.1 343 3UTR 100% 4.050 2.835 N GBA3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_102356.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03849 pDONR223 100% 39.9% None 1_79del;526A>T;566_1214del n/a
2 ccsbBroad304_03849 pLX_304 0% 39.9% V5 1_79del;526A>T;566_1214del n/a
3 TRCN0000478720 ACGTCCCGCTACGTATGTTGGGCA pLX_317 61.2% 39.9% V5 1_79del;526A>T;566_1214del n/a
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