Construct: ORF TRCN0000478732
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015714.1_s317c1
- Derived from:
- ccsbBroadEn_06186
- DNA Barcode:
- AGATCCAATCCACGGGGCCACCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- F2R (2149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478732
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2149 | F2R | coagulation factor II throm... | NM_001992.5 | 99.9% | 99.7% | 1004C>T |
| 2 | human | 2149 | F2R | coagulation factor II throm... | NM_001311313.2 | 71.4% | 71.2% | 0_1ins363;641C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1341
- ORF length:
- 1275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gccgcggcgg ctgctgctgg tggccgcctg cttcagtctg tgcggcccgc 121 tgttgtctgc ccgcacccgg gcccgcaggc cagaatcaaa agcaacaaat gccaccttag 181 atccccggtc atttcttctc aggaacccca atgataaata tgaaccattt tgggaggatg 241 aggagaaaaa tgaaagtggg ttaactgaat acagattagt ctccatcaat aaaagcagtc 301 ctcttcaaaa acaacttcct gcattcatct cagaagatgc ctccggatat ttgaccagct 361 cctggctgac actctttgtc ccatctgtgt acaccggagt gtttgtagtc agcctcccac 421 taaacatcat ggccatcgtt gtgttcatcc tgaaaatgaa ggtcaagaag ccggcggtgg 481 tgtacatgct gcacctggcc acggcagatg tgctgtttgt gtctgtgctc ccctttaaga 541 tcagctatta cttttccggc agtgattggc agtttgggtc tgaattgtgt cgcttcgtca 601 ctgcagcatt ttactgtaac atgtacgcct ctatcttgct catgacagtc ataagcattg 661 accggtttct ggctgtggtg tatcccatgc agtccctctc ctggcgtact ctgggaaggg 721 cttccttcac ttgtctggcc atctgggctt tggccatcgc aggggtagtg cctctgctcc 781 tcaaggagca aaccatccag gtgcccgggc tcaacatcac tacctgtcat gatgtgctca 841 atgaaaccct gctcgaaggc tactatgcct actacttctc agccttctct gctgtcTTCT 901 TTTTTGTGCC GCTGATCATT TCCACGGTCT GTTATGTGTC TATCATTCGA TGTCTTAGCT 961 CTTCCGCAGT TGCCAACCGC AGCAAGAAGT CCCGGGCTTT GTTCCTGTCA GCTGCTGTTT 1021 TCTGCATCTT CATCATTTGC TTCGGACCCA CAAACGTCCT CCTGATTGTG CATTACTCAT 1081 TCCTTTCTCA CACTTCCACC ACAGAGGCTG CCTACTTTGC CTACCTCCTC TGTGTCTGTG 1141 TCAGCAGCAT AAGCTGCTGC ATCGACCCCC TAATTTACTA TTACGCTTCC TCTGAGTGCC 1201 AGAGGTACGT CTACAGTATC TTATGCTGCA AAGAAAGTTC CGATCCCAGC AGTTATAACA 1261 GCAGTGGGCA GTTGATGGCA AGTAAAATGG ATACCTGCTC TAGTAACCTG AATAACAGCA 1321 TATACAAAAA GCTGTTAACT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1381 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1441 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGAT CCAATCCACG 1501 GGGCCACCTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt