Transcript: Human NM_001992.5

Homo sapiens coagulation factor II thrombin receptor (F2R), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-26
Taxon:
Homo sapiens (human)
Gene:
F2R (2149)
Length:
3727
CDS:
183..1460

Additional Resources:

NCBI RefSeq record:
NM_001992.5
NBCI Gene record:
F2R (2149)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001992.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000003688 GACGGCAAGGTTTAAGTTATT pLKO.1 1990 3UTR 100% 13.200 18.480 N F2R n/a
2 TRCN0000220246 CCTGAATAACAGCATATACAA pLKO.1 1424 CDS 100% 5.625 3.938 N F2r n/a
3 TRCN0000003691 CCTACTACTTCTCAGCCTTCT pLKO.1 985 CDS 100% 4.050 2.835 N F2R n/a
4 TRCN0000342352 CCTACTACTTCTCAGCCTTCT pLKO_005 985 CDS 100% 4.050 2.835 N F2R n/a
5 TRCN0000003690 GCATTACTCATTCCTTTCTCA pLKO.1 1187 CDS 100% 3.000 2.100 N F2R n/a
6 TRCN0000352674 GCATTACTCATTCCTTTCTCA pLKO_005 1187 CDS 100% 3.000 2.100 N F2R n/a
7 TRCN0000003689 CCCGGTCATTTCTTCTCAGGA pLKO.1 301 CDS 100% 2.640 1.848 N F2R n/a
8 TRCN0000342351 CCCGGTCATTTCTTCTCAGGA pLKO_005 301 CDS 100% 2.640 1.848 N F2R n/a
9 TRCN0000003687 CCCACAAACGTCCTCCTGATT pLKO.1 1164 CDS 100% 4.950 2.970 N F2R n/a
10 TRCN0000352793 CCCACAAACGTCCTCCTGATT pLKO_005 1164 CDS 100% 4.950 2.970 N F2R n/a
11 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3168 3UTR 100% 4.950 2.475 Y ERAP2 n/a
12 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3169 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001992.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489554 TTGCCCCCCACTAGAATATATGTC pLX_317 25.1% 100% 100% V5 (not translated due to prior stop codon) n/a
2 TRCN0000487842 AAAATATTATGTTCCTGCAGCCCA pLX_317 22.3% 99.9% 99.7% V5 1275_1276insG n/a
3 ccsbBroadEn_06186 pDONR223 100% 99.9% 99.7% None 1004C>T n/a
4 ccsbBroad304_06186 pLX_304 0% 99.9% 99.7% V5 1004C>T n/a
5 TRCN0000478732 AGATCCAATCCACGGGGCCACCTG pLX_317 35.1% 99.9% 99.7% V5 1004C>T n/a
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