Transcript: Human NM_001311313.2

Homo sapiens coagulation factor II thrombin receptor (F2R), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-07-16
Taxon:
Homo sapiens (human)
Gene:
F2R (2149)
Length:
3849
CDS:
668..1582

Additional Resources:

NCBI RefSeq record:
NM_001311313.2
NBCI Gene record:
F2R (2149)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001311313.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000003688 GACGGCAAGGTTTAAGTTATT pLKO.1 2112 3UTR 100% 13.200 18.480 N F2R n/a
2 TRCN0000220246 CCTGAATAACAGCATATACAA pLKO.1 1546 CDS 100% 5.625 3.938 N F2r n/a
3 TRCN0000003691 CCTACTACTTCTCAGCCTTCT pLKO.1 1107 CDS 100% 4.050 2.835 N F2R n/a
4 TRCN0000342352 CCTACTACTTCTCAGCCTTCT pLKO_005 1107 CDS 100% 4.050 2.835 N F2R n/a
5 TRCN0000003690 GCATTACTCATTCCTTTCTCA pLKO.1 1309 CDS 100% 3.000 2.100 N F2R n/a
6 TRCN0000352674 GCATTACTCATTCCTTTCTCA pLKO_005 1309 CDS 100% 3.000 2.100 N F2R n/a
7 TRCN0000003689 CCCGGTCATTTCTTCTCAGGA pLKO.1 423 5UTR 100% 2.640 1.848 N F2R n/a
8 TRCN0000342351 CCCGGTCATTTCTTCTCAGGA pLKO_005 423 5UTR 100% 2.640 1.848 N F2R n/a
9 TRCN0000003687 CCCACAAACGTCCTCCTGATT pLKO.1 1286 CDS 100% 4.950 2.970 N F2R n/a
10 TRCN0000352793 CCCACAAACGTCCTCCTGATT pLKO_005 1286 CDS 100% 4.950 2.970 N F2R n/a
11 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3290 3UTR 100% 4.950 2.475 Y ERAP2 n/a
12 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3291 3UTR 100% 13.200 6.600 Y LIAS n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001311313.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489554 TTGCCCCCCACTAGAATATATGTC pLX_317 25.1% 71.5% 71.5% V5 (not translated due to prior stop codon) 0_1ins363 n/a
2 TRCN0000487842 AAAATATTATGTTCCTGCAGCCCA pLX_317 22.3% 71.4% 71.3% V5 0_1ins363;912_913insG n/a
3 ccsbBroadEn_06186 pDONR223 100% 71.4% 71.2% None 0_1ins363;641C>T n/a
4 ccsbBroad304_06186 pLX_304 0% 71.4% 71.2% V5 0_1ins363;641C>T n/a
5 TRCN0000478732 AGATCCAATCCACGGGGCCACCTG pLX_317 35.1% 71.4% 71.2% V5 0_1ins363;641C>T n/a
Download CSV