Construct: ORF TRCN0000478762
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001349.1_s317c1
- Derived from:
- ccsbBroadEn_15783
- DNA Barcode:
- ATAGTATCGTGCCTACATCAGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGS22 (26166)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478762
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26166 | RGS22 | regulator of G protein sign... | XM_011516959.3 | 67.9% | 67.8% | 391G>C;909A>G;1513_2223del |
| 2 | human | 26166 | RGS22 | regulator of G protein sign... | XM_005250860.3 | 61% | 61% | 391G>C;909A>G;1513_2472del |
| 3 | human | 26166 | RGS22 | regulator of G protein sign... | XM_017013310.2 | 43.5% | 43.4% | 391G>C;909A>G;1513_3471del |
| 4 | human | 26166 | RGS22 | regulator of G protein sign... | XM_017013309.2 | 43.4% | 43.4% | 391G>C;909A>G;1513_3474del |
| 5 | human | 26166 | RGS22 | regulator of G protein sign... | XM_005250858.3 | 41.5% | 41.5% | 391G>C;909A>G;1513_3630del |
| 6 | human | 26166 | RGS22 | regulator of G protein sign... | XM_005250856.3 | 41.5% | 41.5% | 391G>C;909A>G;1513_3633del |
| 7 | human | 26166 | RGS22 | regulator of G protein sign... | XM_005250857.3 | 41.5% | 41.5% | 391G>C;909A>G;1513_3633del |
| 8 | human | 26166 | RGS22 | regulator of G protein sign... | NM_015668.5 | 39.8% | 39.7% | 391G>C;909A>G;1513_3792del |
| 9 | human | 26166 | RGS22 | regulator of G protein sign... | NM_001286692.2 | 38.8% | 38.8% | (many diffs) |
| 10 | human | 26166 | RGS22 | regulator of G protein sign... | XM_006716540.2 | 32.2% | 32.1% | (many diffs) |
| 11 | human | 26166 | RGS22 | regulator of G protein sign... | XM_011516957.1 | 32.2% | 32.1% | (many diffs) |
| 12 | human | 26166 | RGS22 | regulator of G protein sign... | XM_011516958.1 | 32.2% | 32.1% | (many diffs) |
| 13 | human | 26166 | RGS22 | regulator of G protein sign... | NM_001286693.2 | 25.5% | 25.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1578
- ORF length:
- 1512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgagaagagg ctcaccgcgg agccaccaac tattacagaa gaagaatttg 121 aagattctct ggcaacagat gatttccttg tagactactt taatgaattc ctaagccttc 181 caaccttttc agaggcaatt agatttaatg cagattatgg agtttttgaa gtagctaatg 241 atgctccaca atttctggaa aaacaactga aaaaaattct acaaaaccag caacctcgaa 301 atcccattta tgatgttgta aggaaaggaa agaatgaggt taaacctgtt caaatgaatg 361 cccccgatga agatgagacc attaatgtca actacaatat tatgtgtctc agtcgtgaag 421 aaggtattaa gtggatcaaa aaagaaagac ttccaccatt tctggaaagt gattgttact 481 ttgaatacag gttagccaaa ttggtctcac aagtaagatg gagcaagtcc ggcatgaatt 541 tcacagtagg atcaaatttt tctccctgga tcgtgaaaaa accacccagt ctaccacctc 601 ctgccactga agaagataat cttgtaatta tgaaaaagtt ctatgtatca cttggtgagg 661 cttcctatac tcaaacaaaa gattggtttg cattagcaaa acaaagtcag caaacagtat 721 caaccttttc gttaccctgt tgtgtaccct acaacaaact taaatcacca gctatttcat 781 ctgtttctga gaattttatc tttgatgatg gagttcaccc taggacaaaa aaggacccat 841 ctaaaaccaa caaattgatt tctgaatttg aagaagaaga aggagaagaa gaagaggtgt 901 ctgtatctct acaagacact ccttctcaag ctcttctgag agtatacctt gaaaagaaac 961 aggatgttga tgagagcctg acaatgcatt tctcaacatg tgaagaattt ttaagctcct 1021 atatatactt tattttgaga ggagcaattc agcaaattgt tggaaagcca gttggagaaa 1081 ccccagacta tataaacttc aacaatataa caaaagtgtc atttgatgat tgttttgagt 1141 ctattcatGG CAAGAATTTT TTAAGTGAAT TAGTTCAAAC TACAAAGGAG AGGTCAGAAG 1201 AGATAGAACA AACAAGTTTA AGTTCAAAGA ATGAGAGCGC TGGACCAGAG AGCAGGGCGG 1261 ACTGGTGTAT TTCTCATAGG ACTTATGACA TTGGCAATAG AAAGGAGTTT GAAAGATTTA 1321 AGAAATTTAT AAAAGGTACA TTGGGAGAGA GATATTGGTG GCTATGGATG GATATTGAGA 1381 GGTTAAAAGT TCTTAAGGAT CCTGGAAGAC ATCAAAGACA TCTTGAGAAG ATGAAAAAAT 1441 GCTATCTAGT GAGCAATGGA GATTACTACC TTTCTGCAGA AATCTTATCA AAATTTAAAC 1501 TGTTAGATGG ATCACAGTGG AATGAAGAGC ATTTAAGAAA TATTCAGTCT GAAGTTTTAA 1561 AACCTTTACT TCTGTATTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1621 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1681 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATAGTAT CGTGCCTACA 1741 TCAGGTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt