Transcript: Human XM_006716540.2

PREDICTED: Homo sapiens regulator of G protein signaling 22 (RGS22), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RGS22 (26166)
Length:
4303
CDS:
598..4104

Additional Resources:

NCBI RefSeq record:
XM_006716540.2
NBCI Gene record:
RGS22 (26166)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006716540.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423772 GTATGCAAGCAAGCGCAATAT pLKO_005 2458 CDS 100% 13.200 18.480 N RGS22 n/a
2 TRCN0000148118 GCAATGGAGATTACTACCTTT pLKO.1 1697 CDS 100% 4.950 3.960 N RGS22 n/a
3 TRCN0000219846 AGTCGTGAAGAAGGTATTAAG pLKO.1 655 CDS 100% 13.200 9.240 N RGS22 n/a
4 TRCN0000427050 ATTGAGCAGTTCCGGAGAATA pLKO_005 2962 CDS 100% 13.200 9.240 N RGS22 n/a
5 TRCN0000413629 GATGGATATTGAGAGGTTAAA pLKO_005 1611 CDS 100% 13.200 9.240 N RGS22 n/a
6 TRCN0000147225 GAGTCTATTCATGGCAAGAAT pLKO.1 1381 CDS 100% 5.625 3.938 N RGS22 n/a
7 TRCN0000147377 GCAACAGATGATTTCCTTGTA pLKO.1 266 5UTR 100% 4.950 3.465 N RGS22 n/a
8 TRCN0000428326 GAACAGAATATTGGGATAATG pLKO_005 2819 CDS 100% 13.200 7.920 N RGS22 n/a
9 TRCN0000134370 GAAGAAGAAGAAGGAGAAGAA pLKO.1 1114 CDS 100% 4.950 2.475 Y TNFAIP2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006716540.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07998 pDONR223 100% 92.3% 92.2% None (many diffs) n/a
2 ccsbBroad304_07998 pLX_304 0% 92.3% 92.2% V5 (many diffs) n/a
3 TRCN0000492324 AAATACGGAGCCGCTGTGTGCGCC pLX_317 12.2% 92.3% 92.2% V5 (many diffs) n/a
4 ccsbBroadEn_15783 pDONR223 0% 32.2% 32.1% None (many diffs) n/a
5 ccsbBroad304_15783 pLX_304 0% 32.2% 32.1% V5 (many diffs) n/a
6 TRCN0000478762 ATAGTATCGTGCCTACATCAGGTA pLX_317 28.7% 32.2% 32.1% V5 (many diffs) n/a
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