Construct: ORF TRCN0000478764
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000747.1_s317c1
- Derived from:
- ccsbBroadEn_01173
- DNA Barcode:
- CGAGGCTAAAACTTCAGATCCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PEX7 (5191)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478764
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5191 | PEX7 | peroxisomal biogenesis fact... | NM_000288.4 | 100% | 100% | |
| 2 | human | 5191 | PEX7 | peroxisomal biogenesis fact... | XM_005267019.4 | 87.5% | 86.6% | (many diffs) |
| 3 | human | 5191 | PEX7 | peroxisomal biogenesis fact... | XM_006715502.2 | 69.6% | 69.6% | 336_337ins294 |
| 4 | human | 5191 | PEX7 | peroxisomal biogenesis fact... | XM_017010934.2 | 61.6% | 53.9% | 524_525ins277;597_598ins95 |
| 5 | mouse | 18634 | Pex7 | peroxisomal biogenesis fact... | NM_008822.2 | 87.3% | 90.4% | (many diffs) |
| 6 | mouse | 18634 | Pex7 | peroxisomal biogenesis fact... | NM_001161825.1 | 79.5% | 82.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgcggtgtgc ggtggagcgg cgcggatgct gcggacgccg ggacgccacg 121 gctacgccgc cgagttctcc ccgtacctgc cgggccgcct ggcctgcgcc accgcgcagc 181 actacggcat cgcgggctgt ggaaccctac taatattgga tccagatgaa gctgggctaa 241 ggctttttag aagctttgac tggaatgatg gtttgtttga tgtgacttgg agtgagaaca 301 acgaacatgt cctcatcacc tgtagtggcg atggctcgct gcagctctgg gacactgcca 361 aagctgcagg gccactgcaa gtctataaag aacacgctca ggaggtgtat agtgttgatt 421 ggagccaaac cagaggtgaa cagcttgtgg tgtctggctc atgggatcaa actgtcaaat 481 tgtgggatcc aactgttgga aagtctctgt gcacctttag aggccatgaa agtattattt 541 atagcacaat ctggtctccc cacatccctg gttgttttgc ttcagccTCA GGTGATCAGA 601 CTCTGAGAAT ATGGGATGTG AAGGCAGCAG GAGTAAGAAT CGTGATTCCT GCACATCAGG 661 CAGAAATCTT GAGTTGTGAC TGGTGTAAAT ACAATGAGAA TTTGCTGGTG ACCGGGGCGG 721 TTGACTGTAG TTTGAGAGGC TGGGACTTAA GGAATGTACG ACAACCAGTG TTTGAACTTC 781 TTGGTCATAC CTATGCTATT AGGAGGGTGA AATTTTCACC ATTTCATGCT TCTGTGCTGG 841 CCTCTTGCTC GTATGATTTT ACTGTAAGAT TCTGGAACTT TTCAAAGCCT GACTCTCTTC 901 TTGAAACAGT GGAGCATCAT ACAGAGTTTA CTTGTGGTTT AGACTTCAGT CTTCAGAGCC 961 CCACTCAGGT GGCTGACTGT TCTTGGGATG AAACAATAAA GATCTATGAC CCTGCTTGTC 1021 TTACTATTCC TGCTTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGAGGCTAAA ACTTCAGATC 1201 CCCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt