Construct: ORF TRCN0000478767
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018009.1_s317c1
- Derived from:
- ccsbBroadEn_00118
- DNA Barcode:
- CGTGACGAGGATAACACTACGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATIC (471)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478767
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 471 | ATIC | 5-aminoimidazole-4-carboxam... | NM_004044.7 | 100% | 100% | |
| 2 | human | 471 | ATIC | 5-aminoimidazole-4-carboxam... | XM_017004187.2 | 93.6% | 93.4% | (many diffs) |
| 3 | human | 471 | ATIC | 5-aminoimidazole-4-carboxam... | XM_024452919.1 | 90% | 90% | 0_1ins177 |
| 4 | mouse | 108147 | Atic | 5-aminoimidazole-4-carboxam... | NM_026195.3 | 85.7% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1842
- ORF length:
- 1776
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcccggccag ctcgccttat ttagtgtctc tgacaaaacc ggccttgtgg 121 aatttgcaag aaacctgacc gctcttggtt tgaatctggt cgcttccgga gggactgcaa 181 aagctctcag ggatgctggt ctggcagtca gagatgtctc tgagttgacg ggatttcctg 241 aaatgttggg gggacgtgtg aaaactttgc atcctgcagt ccatgctgga atcctagctc 301 gtaatattcc agaagataat gctgacatgg ccagacttga tttcaatctt ataagagttg 361 ttgcctgcaa tctctatccc tttgtaaaga cagtggcttc tccaggtgta actgttgagg 421 aggctgtgga gcaaattgac attggtggag taaccttact gagagctgca gccaaaaacc 481 acgctcgagt gacagtggtg tgtgaaccag aggactatgt ggtggtgtcc acggagatgc 541 agagctccga gagtaaggac acctccttgg agactagacg ccagttagcc ttgaaggcat 601 tcactcatac ggcacaatat gatgaagcaa tttcagatta tttcaggaaa cagtacagca 661 aaggcgtatc tcagatgccc ttgagatatg gaatgaaccc acatcagacc cctgcccagc 721 tgtacacact gcagcccaag cttcccatca cagttctaaa tggagcccct ggatttataa 781 acttgtgcga tgctttgaac gcctggcagc tggtgaagga actcaaggag gctttaggta 841 ttccagccgc tgcctctttc aaacatgtca gcccagcagg tgctgctgtt ggaattccac 901 tcagtgaaga tgaggccaaa gtctgcatgg tttatgatct ctataaaacc ctcacaccca 961 tctcagcggc atatgcaaga gcaagagggg ctgataggat gtcttcattt ggtgattttg 1021 ttgcattgtc cgatgtttgt gatgtaccaa ctgcaaaaat tatttccaga gaagtatctg 1081 atggtataat tgccccagga tatgaagaag aagccttgac aatactttcc aaaaagaaaa 1141 atggaaacta ttgtgtcctt cagatggacc aatcttacaa accagatgaa aatgaagttc 1201 gaactctctt tggtcttcat ttaagccaga agagaaataa tggtgtcgtc gacaagtcat 1261 tatttagcaa tgttgttacc aaaaataaag atttgccaga gtctgccctc cgagacctca 1321 tcgtagccac cattgctgtc aagtacactc agtctaactc tgtgtgctac gccaagaacg 1381 ggcaggttat cggCATTGGA GCAGGACAGC AGTCTCGTAT ACACTGCACT CGCCTTGCAG 1441 GAGATAAGGC AAACTATTGG TGGCTTAGAC ACCATCCACA AGTGCTTTCG ATGAAGTTTA 1501 AAACAGGAGT GAAGAGAGCA GAAATCTCCA ATGCCATCGA TCAATATGTG ACTGGAACCA 1561 TTGGCGAGGA TGAAGATTTG ATAAAGTGGA AGGCACTGTT TGAGGAAGTC CCTGAGTTAC 1621 TCACTGAGGC AGAGAAGAAG GAATGGGTTG AGAAACTGAC TGAAGTTTCT ATCAGCTCTG 1681 ATGCCTTCTT CCCTTTCCGA GATAACGTAG ACAGAGCTAA AAGGAGTGGT GTGGCGTACA 1741 TTGCGGCTCC CTCCGGTTCT GCTGCTGACA AAGTTGTGAT TGAGGCCTGC GACGAACTGG 1801 GAATCATCCT CGCTCATACG AACCTTCGGC TCTTCCACCA CTGCCCAACT TTCTTGTACA 1861 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1921 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1981 AGGACGACGT GACGAGGATA ACACTACGGG AACGCGTTAA GTCgacaatc aacctctgga 2041 ttacaaaatt tgtgaaagat t