Transcript: Human XM_024452919.1

PREDICTED: Homo sapiens 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ATIC (471)
Length:
2183
CDS:
473..2074

Additional Resources:

NCBI RefSeq record:
XM_024452919.1
NBCI Gene record:
ATIC (471)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024452919.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000034967 GCAATCTCTATCCCTTTGTAA pLKO.1 597 CDS 100% 5.625 4.500 N ATIC n/a
2 TRCN0000311707 GCAATCTCTATCCCTTTGTAA pLKO_005 597 CDS 100% 5.625 4.500 N ATIC n/a
3 TRCN0000034968 GCTGGAATCCTAGCTCGTAAT pLKO.1 515 CDS 100% 10.800 7.560 N ATIC n/a
4 TRCN0000311706 GCTGGAATCCTAGCTCGTAAT pLKO_005 515 CDS 100% 10.800 7.560 N ATIC n/a
5 TRCN0000034966 GCAGGAGATAAGGCAAACTAT pLKO.1 1667 CDS 100% 5.625 3.938 N ATIC n/a
6 TRCN0000311720 GCAGGAGATAAGGCAAACTAT pLKO_005 1667 CDS 100% 5.625 3.938 N ATIC n/a
7 TRCN0000034965 CGGCACAATATGATGAAGCAA pLKO.1 840 CDS 100% 3.000 2.100 N ATIC n/a
8 TRCN0000034964 GCCTTGACAATACTTTCCAAA pLKO.1 1343 CDS 100% 4.950 2.970 N ATIC n/a
9 TRCN0000311632 GCCTTGACAATACTTTCCAAA pLKO_005 1343 CDS 100% 4.950 2.970 N ATIC n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024452919.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00118 pDONR223 100% 90% 90% None 0_1ins177 n/a
2 ccsbBroad304_00118 pLX_304 0% 90% 90% V5 0_1ins177 n/a
3 TRCN0000478767 CGTGACGAGGATAACACTACGGGA pLX_317 25.5% 90% 90% V5 0_1ins177 n/a
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