Construct: ORF TRCN0000478768
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017228.2_s317c1
- Derived from:
- ccsbBroadEn_04977
- DNA Barcode:
- GCACCCATTTAACACCACGAGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MDP1 (145553)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478768
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145553 | MDP1 | magnesium dependent phospha... | NM_138476.4 | 100% | 100% | |
| 2 | human | 100528064 | NEDD8-MDP1 | NEDD8-MDP1 readthrough | NM_001199823.2 | 81.1% | 77.2% | (many diffs) |
| 3 | human | 145553 | MDP1 | magnesium dependent phospha... | NM_001199822.1 | 77.6% | 76.7% | 406_406delAinsGTTAC;409_410insCATTCACATCC;411_412ins102 |
| 4 | human | 145553 | MDP1 | magnesium dependent phospha... | NM_001199821.1 | 69.3% | 45.2% | 262_263ins140;366_367ins22 |
| 5 | human | 100528064 | NEDD8-MDP1 | NEDD8-MDP1 readthrough | NR_137630.1 | 51.9% | (many diffs) | |
| 6 | human | 100528064 | NEDD8-MDP1 | NEDD8-MDP1 readthrough | NR_137631.1 | 49.9% | (many diffs) | |
| 7 | human | 100528064 | NEDD8-MDP1 | NEDD8-MDP1 readthrough | NR_137632.1 | 38.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcggctaccg aagctggcag tctttgattt ggattacact ctctggcctt 121 tctgggtcga cacgcacgta gaccctccgt tccataagag cagtgatgga actgtacgag 181 ataggcgggg ccaagacgtc cgactgtacc cagaggtgcc TGAGGTCCTA AAACGATTGC 241 AGAGCCTTGG GGTGCCCGGT GCGGCTGCTT CAAGGACAAG TGAGATAGAA GGGGCCAACC 301 AGCTACTGGA GCTCTTTGAC CTCTTCAGGT ACTTTGTTCA TCGGGAAATC TATCCAGGCA 361 GCAAGATCAC ACACTTTGAG AGGTTGCAGC AGAAGACTGG AATTCCTTTC TCCCAGATGA 421 TCTTCTTTGA TGATGAGAGG CGGAATATTG TAGACGTCAG CAAACTGGGT GTTACCTGCA 481 TTCACATCCA GAATGGAATG AATCTTCAAA CTCTAAGTCA AGGGTTAGAG ACATTTGCGA 541 AGGCCCAAAC TGGGCCTTTG AGGTCCAGCC TTGAGGAGAG CCCATTTGAG GCCTACCCAA 601 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 661 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 721 TATCTTGTGG AAAGGACGAG CACCCATTTA ACACCACGAG ACAACGCGTT AAGTCgacaa 781 tcaacctctg gattacaaaa tttgtgaaag att