Construct: ORF TRCN0000478784
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003138.1_s317c1
- Derived from:
- ccsbBroadEn_04508
- DNA Barcode:
- CAAAATCATATTTAGTCCCTGAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UCN2 (90226)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478784
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90226 | UCN2 | urocortin 2 | NM_033199.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 426
- ORF length:
- 336
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGACCAGGTG TGCTCTGCTG TTGCTGATGG 121 TCCTGATGTT GGGCAGAGTC CTGGTTGTCC CAGTGACCCC TATCCCAACC TTCCAGCTCC 181 GCCCTCAGAA TTCTCCCCAG ACCACTCCCC GACCTGCGGC CTCAGAGAGC CCCTCAGCTG 241 CTCCCACATG GCCGTGGGCT GCCCAGAGCC ACTGCAGCCC CACCCGCCAC CCTGGCTCGC 301 GCATTGTCCT ATCGCTGGAT GTCCCCATCG GCCTCTTGCA GATCTTACTG GAGCAAGCCC 361 GGGCCAGGGC TGCCAGGGAG CAGGCCACCA CCAACGCCCG CATCCTGGCC CGTGTCGGCC 421 ACTGCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 481 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGCCCGTA ACTTGAAAGT ATTTCGATTT 541 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACAAAATCAT ATTTAGTCCC TGAAGACGCG 601 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt