Construct: ORF TRCN0000478795
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012236.1_s317c1
- Derived from:
- ccsbBroadEn_03668
- DNA Barcode:
- AAGAGCCGGCCCCCTCATGCAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSENEN (55851)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478795
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55851 | PSENEN | presenilin enhancer, gamma-... | NM_001281532.3 | 100% | 100% | |
| 2 | human | 55851 | PSENEN | presenilin enhancer, gamma-... | NM_172341.4 | 100% | 100% | |
| 3 | mouse | 66340 | Psenen | presenilin enhancer gamma s... | NM_025498.2 | 91% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 393
- ORF length:
- 303
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAACCTGGA GCGAGTGTCC AATGAGGAGA 121 AATTGAACCT GTGCCGGAAG TACTACCTGG GGGGGTTTGC TTTCCTGCCT TTTCTCTGGT 181 TGGTCAACAT CTTCTGGTTC TTCCGAGAGG CCTTCCTTGT CCCAGCCTAC ACAGAACAGA 241 GCCAAATCAA AGGCTATGTC TGGCGCTCAG CTGTGGGCTT CCTCTTCTGG GTGATAGTGC 301 TCACCTCCTG GATCACCATC TTCCAGATCT ACCGGCCCCG CTGGGGTGCC CTTGGGGACT 361 ACCTCTCCTT CACCATACCC CTGGGCACCC CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 541 GAGCCGGCCC CCTCATGCAA TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt