Construct: ORF TRCN0000478806
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015032.1_s317c1
- Derived from:
- ccsbBroadEn_03380
- DNA Barcode:
- GCCCACGTACTTGGGCGTTCCTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB8B (51762)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478806
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51762 | RAB8B | RAB8B, member RAS oncogene ... | NM_016530.3 | 100% | 100% | |
| 2 | human | 51762 | RAB8B | RAB8B, member RAS oncogene ... | XM_017022312.1 | 60.8% | 60.8% | 0_1ins243 |
| 3 | human | 51762 | RAB8B | RAB8B, member RAS oncogene ... | XM_011521669.3 | 48.4% | 36.9% | (many diffs) |
| 4 | mouse | 235442 | Rab8b | RAB8B, member RAS oncogene ... | NM_173413.3 | 92.5% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 687
- ORF length:
- 621
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaagacgtac gattatctct tcaagctcct gctgatcggc gactcggggg 121 taggcaagac ctgcctcctg ttccgcttct cagaggacgc cttcaacacc accttcatct 181 ccaccatcgg aattgatttt aaaattagaa cgatagaact agatggaaag aaaattaagc 241 ttcagatatg ggacacagcg ggtcaggaaa gattccgaac aatcacgaca gcgtactaca 301 gaggagccat gggcattatg ctggtctatg acatCACAAA TGAAAAATCC TTTGACAATA 361 TTAAAAATTG GATCAGAAAC ATTGAAGAGC ATGCCTCTTC CGATGTCGAA AGAATGATCC 421 TGGGTAACAA ATGTGATATG AATGACAAAA GACAAGTGTC AAAAGAAAGA GGGGAGAAGC 481 TAGCAATTGA CTATGGGATT AAATTCTTGG AGACAAGCGC AAAATCCAGT GCAAATGTAG 541 AAGAGGCATT TTTTACACTT GCACGAGATA TAATGACAAA ACTCAACAGA AAAATGAATG 601 ACAGCAATTC AGCAGGAGCA GGTGGACCAG TGAAAATAAC AGAAAACCGA TCAAAGAAGA 661 CCAGTTTCTT TCGTTGCTCG CTACTTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 721 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 781 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCCACGT 841 ACTTGGGCGT TCCTCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 901 aagatt