Construct: ORF TRCN0000478810
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008111.1_s317c1
- Derived from:
- ccsbBroadEn_12367
- DNA Barcode:
- TGGGCCATCACTGCGTGATTTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP20 (57569)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478810
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57569 | ARHGAP20 | Rho GTPase activating prote... | XM_005271628.3 | 99% | 99% | (many diffs) |
| 2 | human | 57569 | ARHGAP20 | Rho GTPase activating prote... | NM_001258417.1 | 62.9% | 62.9% | (many diffs) |
| 3 | human | 57569 | ARHGAP20 | Rho GTPase activating prote... | NM_001258418.1 | 62.9% | 62.9% | (many diffs) |
| 4 | human | 57569 | ARHGAP20 | Rho GTPase activating prote... | NM_001258416.1 | 62.4% | 62.4% | (many diffs) |
| 5 | human | 57569 | ARHGAP20 | Rho GTPase activating prote... | NM_001258415.2 | 62.2% | 62.2% | (many diffs) |
| 6 | human | 57569 | ARHGAP20 | Rho GTPase activating prote... | NM_020809.3 | 61% | 61% | (many diffs) |
| 7 | human | 57569 | ARHGAP20 | Rho GTPase activating prote... | XM_006718893.3 | 61% | 61% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2259
- ORF length:
- 2193
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tcaaggaaat gatgaagaga aaataaatac tgttcaaagg ctattagacc 121 agcttccgag agccaatgtt gttctcctaa ggtatctttt tggggtgtta cacaacattg 181 agcaacattc ctcatccaat cagatgactg catttaattt agctgtgtgt gtcgctccaa 241 gtattctttg gcctcctgct tcctccagcc cagaactaga aaacgaattt acaaaaaagg 301 tttccctgct tatacaattt ctgattgaga attgccttag gatatttgga gaagaaatca 361 cttccctctt cagagaggtt tcagtgagat gtgacactag agagaatgcc tcagatattt 421 cttgctttca actgaatgac tcctcctatg acagcttgga aaatgagcta aatgaggatg 481 ttgatgcacc atgcagtgac ttggtaaaga aacttggcca ggggagcaga agcatggact 541 ctgtcttaac cctcagtgac tatgatcttg accagcccga ggtggaagac cttttaaccc 601 taagcgactt tgacttggcc cattctaaag atgaagatgt tcaaatgaaa cggcctcttg 661 aatccaagcc ggtgaacatt ttagtgtaca caaagatccc actgcgggat catgccaggg 721 ccccatctgc catgtgcaca cccagctacc tgtccacagc tgcagcaaat gctgcaaaaa 781 gcctgaggcg acaccggcgt tgctcagagc ccagcatcga ctatctggat tcaaagcttt 841 cctacctcag ggagttttat cagaaaaagc tacgcaagtc cagctgtgat gcaattcttt 901 ctcaaaaaga tgaagactat ctgaagcaga atcaacccct ccaggaggaa ggaaagacat 961 gttttaaaca gagtttagtc acaggcactg atgtcagcaa gaaaaatgcc actactcaaa 1021 acactaagaa gaaaagcttg tctggtagtg aaggaaatca cgtgaaactt ttccctaagt 1081 ctaagccagt ggccatttct gtggcatctt atagtcctat gtcctcacag gatcattcca 1141 agaaccagcc ctttgatgtg aatacatctg gatactcccc accacacaca gcagatgccc 1201 tcaagggtcc aaggacacat cggcgctgct cagagcccaa catagaagac cagaaccgca 1261 agctgaccta tctcagggga atttattcaa agaaacaaca taaaaccagc tgtgaagctg 1321 gtctcttgca tggagaggag gattatctca aacggcataa gtctttgcaa atggaggggc 1381 agaagctcat taatcagagt ttagtcatgg ggattgaggt gggcaagagt agtgccacaa 1441 accaaaacac tgagaaggtt ttacccccaa gattaaacct ttgcccaagg accagctatt 1501 ccagcttatc ctccccaggc acttccccat ccggctcatc agtaagctcc caagacagtg 1561 ctttttctca gatttctgaa cactctgtgt ttacacccac tgagacttcc tctccaatag 1621 attgcacttt tcaggctcag agaaaacggg aagacctttc tcctgacttt agcaatgcca 1681 gccatgtttc cggaatgccc ggtccctcat cagggcaggc ttgcagccgc ccagcctata 1741 caaagaagga caccatggag tggcattcac aaatgcattc tgtaactctt catcccagca 1801 catggttgag aaatggtgtg gccagtttga aaaactggtc cctcaaaaag aaagcaaagg 1861 cagccagacc agaggaagag aaaatagctt ctccaaaagg acccTTAGAG CCACCCCCAC 1921 ATGCTTCTGG TGTTCCAGAA GCCAACTCAC TGCAAGAGGA ACAAAAAGAC TTGCCCTTAA 1981 GGGCAGCTGA AGGACTGTCC CCTGTGCAGT CAGCCCAAAG GTGTAGTTCT TCTCCCTTCC 2041 AGGACTCAGA GAGACACTGT AGCTCTCCAT TCAGCCTGGT GGAGAGCAGA CTTAAGCTGT 2101 GCATGAAGTC ACATGAGGAA ATAGAGCCTG GTAGTCAGAG CTCTTCTGGT TCTCTGCCTT 2161 GGGAAAGAGC CTCAGCCAGC TCTTGGACTC TAGAGGATGC GACCAGCCCA GACTCAGGGC 2221 CTACAGTGGT CTGCGACATT GAGGACAGAA ACTGCTGCTA CCCAACTTTC TTGTACAAAG 2281 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2341 GAACTAGTCC GTAACTTAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 2401 CGATGGGCCA TCACTGCGTG ATTTACTACG CGTTAAGTCg acaatcaacc tctggattac 2461 aaaatttgtg aaagatt