Transcript: Human NM_001258416.1

Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 3, mRNA.

Source:
NCBI, updated 2018-06-25
Taxon:
Homo sapiens (human)
Gene:
ARHGAP20 (57569)
Length:
6085
CDS:
253..3750

Additional Resources:

NCBI RefSeq record:
NM_001258416.1
NBCI Gene record:
ARHGAP20 (57569)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001258416.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000047227 GCATCGACTATCTGGATTCAA pLKO.1 2294 CDS 100% 5.625 7.875 N ARHGAP20 n/a
2 TRCN0000047226 CCTCTCCAATAGATTGCACTT pLKO.1 3089 CDS 100% 4.050 5.670 N ARHGAP20 n/a
3 TRCN0000423482 GAACAGGAACTGGGTTAATAA pLKO_005 3777 3UTR 100% 15.000 10.500 N ARHGAP20 n/a
4 TRCN0000433901 GAGAATTGCCTTAGGATATTT pLKO_005 1807 CDS 100% 15.000 10.500 N ARHGAP20 n/a
5 TRCN0000047225 CCATCTCTTATCCTGGATAAA pLKO.1 316 CDS 100% 13.200 9.240 N ARHGAP20 n/a
6 TRCN0000047223 CCCAAATGTTTCATGGCTTAT pLKO.1 4911 3UTR 100% 10.800 7.560 N ARHGAP20 n/a
7 TRCN0000047224 GCCCATTCTAAAGATGAAGAT pLKO.1 2098 CDS 100% 4.950 3.465 N ARHGAP20 n/a
8 TRCN0000432933 GACATTGAGGACAGGTATTTA pLKO_005 3715 CDS 100% 15.000 9.000 N ARHGAP20 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001258416.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12367 pDONR223 100% 62.4% 62.4% None (many diffs) n/a
2 ccsbBroad304_12367 pLX_304 0% 62.4% 62.4% V5 (many diffs) n/a
3 TRCN0000478810 TGGGCCATCACTGCGTGATTTACT pLX_317 16.4% 62.4% 62.4% V5 (many diffs) n/a
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