Construct: ORF TRCN0000478811
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006974.1_s317c1
- Derived from:
- ccsbBroadEn_00946
- DNA Barcode:
- ACAATGAGTGTACATAATTTCCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMNA (4000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478811
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4000 | LMNA | lamin A/C | NM_170707.4 | 100% | 100% | |
| 2 | human | 4000 | LMNA | lamin A/C | NM_170708.3 | 95.4% | 95.4% | 1608_1609ins90 |
| 3 | human | 4000 | LMNA | lamin A/C | NM_001282626.2 | 92.4% | 92.4% | 1813_1814ins150 |
| 4 | human | 4000 | LMNA | lamin A/C | NM_001282625.1 | 85.9% | 85.2% | (many diffs) |
| 5 | human | 4000 | LMNA | lamin A/C | NM_005572.3 | 85.9% | 85.2% | (many diffs) |
| 6 | human | 4000 | LMNA | lamin A/C | XM_011509533.1 | 82.7% | 82% | (many diffs) |
| 7 | human | 4000 | LMNA | lamin A/C | NM_001257374.3 | 80% | 79.3% | (many diffs) |
| 8 | human | 4000 | LMNA | lamin A/C | NM_001282624.1 | 72.2% | 66.3% | (many diffs) |
| 9 | human | 4000 | LMNA | lamin A/C | XM_011509534.2 | 65.3% | 53.5% | 0_1ins664;270_309del |
| 10 | human | 4000 | LMNA | lamin A/C | XR_921781.2 | 61.1% | 1_247del;1181_1220del;2280_3257del | |
| 11 | mouse | 16905 | Lmna | lamin A | NM_001002011.3 | 89.9% | 96.3% | (many diffs) |
| 12 | mouse | 16905 | Lmna | lamin A | XM_006501073.1 | 89.9% | 96.3% | (many diffs) |
| 13 | mouse | 16905 | Lmna | lamin A | NM_001111102.2 | 77.1% | 82.9% | (many diffs) |
| 14 | mouse | 16905 | Lmna | lamin A | NM_019390.3 | 61.2% | 65.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2058
- ORF length:
- 1992
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaccccgtcc cagcggcgcg ccacccgcag cggggcgcag gccagctcca 121 ctccgctgtc gcccacccgc atcacccggc tgcaggagaa ggaggacctg caggagctca 181 atgatcgctt ggcggtctac atcgaccgtg tgcgctcgct ggaaacggag aacgcagggc 241 tgcgccttcg catcaccgag tctgaagagg tggtcagccg cgaggtgtcc ggcatcaagg 301 ccgcctacga ggccgagctc ggggatgccc gcaagaccct tgactcagta gccaaggagc 361 gcgcccgcct gcagctggag ctgagcaaag tgcgtgagga gtttaaggag ctgaaagcgc 421 gcaataccaa gaaggagggt gacctgatag ctgctcaggc tcggctgaag gacctggagg 481 ctctgctgaa ctccaaggag gccgcactga gcactgctct cagtgagaag cgcacgctgg 541 agggcgagct gcatgatctg cggggccagg tggccaagct tgaggcagcc ctaggtgagg 601 ccaagaagca acttcaggat gagatgctgc ggcgggtgga tgctgagaac aggctgcaga 661 ccatgaagga ggaactggac ttccagaaga acatctacag tgaggagctg cgtgagacca 721 agcgccgtca tgagacccga ctggtggaga ttgacaatgg gaagcagcgt gagtttgaga 781 gccggctggc ggatgcgctg caggaactgc gggcccagca tgaggaccag gtggagcagt 841 ataagaagga gctggagaag acttattctg ccaagctgga caatgccagg cagtctgctg 901 agaggaacag caacctggtg ggggctgccc acgaggagct gcagcagtcg cgcatccgca 961 tcgacagcct ctctgcccag ctcagccagc tccagaagca gctggcagcc aaggaggcga 1021 agcttcgaga cctggaggac tcactggccc gtgagcggga caccagccgg cggctgctgg 1081 cggaaaagga gcgggagatg gccgagatgc gggcaaggat gcagcagcag ctggacgagt 1141 accaggagct tctggacatc aagctggccc tggacatgga gatccacgcc taccgcaagc 1201 tcttggaggg cgaggaggag aggctacgcc tgtcccccag ccctacctcg cagcgcagcc 1261 gtggccgtgc ttcctctcac tcatcccaga cacagggtgg gggcagcgtc accaaaaagc 1321 gcaaactgga gtccactgag agccgcagca gcttctcaca gcacgcacgc actagcgggc 1381 gcgtggccgt ggaggaggtg gatgaggagg gcaagtttgt ccggctgcgc aacaagtcca 1441 atgaggacca gtccatgggc aattggcaga tcaagcgcca gaatggagat gatcccttgc 1501 tgacttaccg gttcccacca aagttcaccc tgaaggctgg gcaggtggtg acgatctggg 1561 ctgcaggagc tggggccacc cacagccccc ctaccgacct ggtgtggaag gcacagaaca 1621 ccTGGGGCTG CGGGAACAGC CTGCGTACGG CTCTCATCAA CTCCACTGGG GAAGAAGTGG 1681 CCATGCGCAA GCTGGTGCGC TCAGTGACTG TGGTTGAGGA CGACGAGGAT GAGGATGGAG 1741 ATGACCTGCT CCATCACCAC CACGGCTCCC ACTGCAGCAG CTCGGGGGAC CCCGCTGAGT 1801 ACAACCTGCG CTCGCGCACC GTGCTGTGCG GGACCTGCGG GCAGCCTGCC GACAAGGCAT 1861 CTGCCAGCGG CTCAGGAGCC CAGGTGGGCG GACCCATCTC CTCTGGCTCT TCTGCCTCCA 1921 GTGTCACGGT CACTCGCAGC TACCGCAGTG TGGGGGGCAG TGGGGGTGGC AGCTTCGGGG 1981 ACAATCTGGT CACCCGCTCC TACCTCCTGG GCAACTCCAG CCCCCGAACC CAGAGCCCCC 2041 AGAACTGCAG CATCATGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2101 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2161 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACAATGA GTGTACATAA 2221 TTTCCGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt