Construct: ORF TRCN0000478813
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006713.1_s317c1
- Derived from:
- ccsbBroadEn_12517
- DNA Barcode:
- TAATAATCCCCTCTGATATATGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2Z (65264)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478813
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65264 | UBE2Z | ubiquitin conjugating enzym... | NM_023079.5 | 69.3% | 69.4% | 1_324del;846C>T |
| 2 | mouse | 268470 | Ube2z | ubiquitin-conjugating enzym... | NM_172300.3 | 65.5% | 69.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 804
- ORF length:
- 738
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc catttataag gagcctcctc caggaatgtt cgttgtacct gatactgttg 121 acatgactaa gattcatgca ttgatcacag gcccatttga cactccttat gaagggggtt 181 tcttcctgtt cgtgtttcgg tgtccgcccg actatcccat ccacccacct cgggtcaaac 241 tgatgacaac gggcaataac acagtgaggt ttaaccccaa cttctaccgc aatgggaaag 301 tctgcttgag tattctaggt acatggactg gacctgcctg gagcccagcc cagagcatct 361 cctcagtgct catctctatc cagtccctga tgactgagaa cccctatcac aatgagcccg 421 gctttGAACA GGAGAGACAT CCAGGAGACA GCAAAAACTA TAATGAATGT ATCCGGCACG 481 AGACCATCAG AGTTGCAGTC TGTGACATGA TGGAAGGAAA GTGTCCCTGT CCTGAACCCC 541 TACGAGGGGT GATGGAGAAG TCCTTTCTGG AGTATTACGA CTTCTATGAG GTGGCCTGCA 601 AAGATCGCCT GCACCTTCAA GGCCAAACTA TGCAGGACCC TTTTGGAGAG AAGCGGGGCC 661 ACTTTGACTA CCAGTCCCTC TTGATGCGCC TGGGACTGAT ACGTCAGAAA GTGCTGGAGA 721 GGCTCCATAA TGAGAATGCA GAAATGGACT CTGATAGCAG TTCATCTGGG ACAGAGACAG 781 ACCTTCATGG GAGCCTGAGG GTTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 841 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGCCCGTAAC 901 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AATAATCCCC 961 TCTGATATAT GACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1021 att