Construct: ORF TRCN0000478814
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004154.1_s317c1
- Derived from:
- ccsbBroadEn_11300
- DNA Barcode:
- ACTTCCCACGAAGTGAAATAATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFSF14 (8740)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478814
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8740 | TNFSF14 | TNF superfamily member 14 | NM_003807.4 | 73.6% | 73.3% | 358C>G;532_720del |
| 2 | human | 8740 | TNFSF14 | TNF superfamily member 14 | XM_017027417.1 | 73.6% | 73.3% | 358C>G;532_720del |
| 3 | human | 8740 | TNFSF14 | TNF superfamily member 14 | XM_017027418.1 | 64.7% | 61.7% | (many diffs) |
| 4 | human | 8740 | TNFSF14 | TNF superfamily member 14 | NM_172014.3 | 58.6% | 58.3% | 111_112ins108;250C>G;424_612del |
| 5 | human | 8740 | TNFSF14 | TNF superfamily member 14 | XR_001753777.1 | 25.3% | (many diffs) | |
| 6 | human | 8740 | TNFSF14 | TNF superfamily member 14 | XR_936212.2 | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 597
- ORF length:
- 531
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagagtgtc gtacggccct cagtgtttgt ggtggatgga cagaccgaca 121 tcccattcac gaggctggga cgaagccacc ggagacagtc gtgcagtgtg gcccgggtgg 181 gtctgggtct cttgctgttg ctgatggggg ccgggctggc cgtccaaggc tggttccTCC 241 TGCAGCTGCA CTGGCGTCTA GGAGAGATGG TCACCCGCCT GCCTGACGGA CCTGCAGGCT 301 CCTGGGAGCA GCTGATACAA GAGCGAAGGT CTCACGAGGT CAACCCAGCA GCGCATCTCA 361 CAGGGGCCAA CTCCAGCTTG ACCGGCAGCG GGGGGCCGCT GTTATGGGAG ACTCAGCTGG 421 GCGTGGCCTT CCTGAGGGGC CTCAGCTACC ACGATGGGGC CCTTGTGGTC ACCAAAGCTG 481 GCTACTACTA CATCTACTCC AAGGTGCAGC TGGGCGGTGT GGGCTGCCCG CTGGGCCTGG 541 CCAGCACCAT CACCCACGGC CTCTACAAGC GCACACCCCG CTACCCCGAG GAGCTGTACC 601 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 661 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 721 ATATATCTTG TGGAAAGGAC GAACTTCCCA CGAAGTGAAA TAATCTACGC GTTAAGTCga 781 caatcaacct ctggattaca aaatttgtga aagatt