Construct: ORF TRCN0000478815
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003885.1_s317c1
- Derived from:
- ccsbBroadEn_08860
- DNA Barcode:
- CGAGCGTTTAGGCCCATGTGTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UNKL (64718)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478815
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64718 | UNKL | unk like zinc finger | NM_001037125.4 | 99.7% | 100% | 12T>C;57T>G |
| 2 | human | 64718 | UNKL | unk like zinc finger | XM_011522613.1 | 68% | 65.1% | (many diffs) |
| 3 | human | 64718 | UNKL | unk like zinc finger | XR_001751965.1 | 40.8% | (many diffs) | |
| 4 | human | 64718 | UNKL | unk like zinc finger | NM_001193388.4 | 39.4% | 35.8% | (many diffs) |
| 5 | human | 64718 | UNKL | unk like zinc finger | XM_011522611.1 | 37.5% | 35.8% | (many diffs) |
| 6 | human | 64718 | UNKL | unk like zinc finger | NM_001372107.1 | 36.7% | 33.4% | (many diffs) |
| 7 | human | 64718 | UNKL | unk like zinc finger | XM_011522610.1 | 35% | 33.5% | (many diffs) |
| 8 | human | 64718 | UNKL | unk like zinc finger | XM_011522612.1 | 25.8% | 24.2% | (many diffs) |
| 9 | human | 64718 | UNKL | unk like zinc finger | NR_160278.1 | 23.1% | (many diffs) | |
| 10 | human | 64718 | UNKL | unk like zinc finger | XR_932901.1 | 22.1% | (many diffs) | |
| 11 | human | 64718 | UNKL | unk like zinc finger | XR_002957834.1 | 20.6% | (many diffs) | |
| 12 | mouse | 74154 | Unkl | unkempt family like zinc fi... | XM_006525022.3 | 35.7% | 36.4% | (many diffs) |
| 13 | mouse | 74154 | Unkl | unkempt family like zinc fi... | XM_006525021.3 | 35.2% | 35.9% | (many diffs) |
| 14 | mouse | 74154 | Unkl | unkempt family like zinc fi... | NM_001197024.1 | 31.5% | 32.2% | (many diffs) |
| 15 | mouse | 74154 | Unkl | unkempt family like zinc fi... | XM_017317696.1 | 27.2% | 27.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 897
- ORF length:
- 831
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gtcggtctcg aaagcggcgg cagcggcgct gagcgggtcc cccccgcaga 121 cggagaagcc gacccactac aggtacctga aggagttcag gacggagcag tgccccctgt 181 tttcacagca caagtgcgcg cagcaccggc cgttcacctg cttccactgg cacttcctca 241 accagcggcg ccgcaggccc ctccgcaggc gcgacggcac cttcaactac agccccgacg 301 tgtactgctc caagtacaac gaagccaccg gcgtgtgccc cgacggcgac gagtgtccct 361 acctgcaccg gacgacgggg gacacagaac gcaagtacca cctgcgttac tacaaaacag 421 gaacctgcat ccacgagaca gacgcacgtg gccactgcgt gaagaatggg ctgcactgtg 481 ccttcgcgca cggccccctg gacctgcggc cgcccgtgtg tgacgtcagg gagctgcagg 541 cccaggaagc cttgcagaac ggccagctgg gcggcgggGA AGGGGTCCCG GATCTGCAGC 601 CTGGGGTCTT GGCCAGCCAG GCCATGATTG AGAAGATCCT GAGCGAGGAC CCCCGGTGGC 661 AAGATGCCAA CTTCGTGCTG GGCAGCTACA AGACGGAGCA GTGCCCGAAG CCGCCACGCC 721 TGTGCCGCCA GGGCTATGCG TGCCCACACT ACCACAATAG CCGGGACAGG CGGCGCAACC 781 CCCGGCGGTT CCAGTACAGC TGGCAGCTGG GACGCCGGGT CCTTAGGCTG AGTCCCAGGG 841 CCAACAACCC AAGGGTCGCC CTGCCCAGGG TGCACACAGG ACCTTCCTCC ACCGCCTGCC 901 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 961 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1021 ATATATCTTG TGGAAAGGAC GACGAGCGTT TAGGCCCATG TGTTTCACGC GTTAAGTCga 1081 caatcaacct ctggattaca aaatttgtga aagatt