Construct: ORF TRCN0000478834
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000146.2_s317c1
- Derived from:
- ccsbBroadEn_02293
- DNA Barcode:
- GGGAACACCACAAGTGTTGCTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNE (10020)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478834
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | NM_005476.7 | 100% | 100% | |
| 2 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | XM_017014167.1 | 100% | 100% | |
| 3 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | NM_001128227.3 | 95.8% | 95.8% | 1_93del |
| 4 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | NM_001190388.1 | 94% | 92.8% | (many diffs) |
| 5 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | XM_017014168.1 | 92.9% | 92.9% | 614_615ins153 |
| 6 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | NM_001190383.3 | 89.7% | 89.7% | 1409_1410ins222 |
| 7 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | XM_005251334.4 | 89.1% | 89.1% | 1_93del;707_708ins153 |
| 8 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | NM_001190384.3 | 84.7% | 84.7% | 0_1ins177;437_438ins153 |
| 9 | mouse | 50798 | Gne | glucosamine (UDP-N-acetyl)-... | NM_001190414.1 | 87.6% | 98.7% | (many diffs) |
| 10 | mouse | 50798 | Gne | glucosamine (UDP-N-acetyl)-... | XM_006538073.3 | 84.7% | 95.4% | (many diffs) |
| 11 | mouse | 50798 | Gne | glucosamine (UDP-N-acetyl)-... | NM_015828.3 | 84% | 94.6% | (many diffs) |
| 12 | mouse | 50798 | Gne | glucosamine (UDP-N-acetyl)-... | XM_006538074.3 | 81% | 90.7% | (many diffs) |
| 13 | mouse | 50798 | Gne | glucosamine (UDP-N-acetyl)-... | XM_017320310.1 | 63.1% | 70.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2235
- ORF length:
- 2166
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagaagaat ggaaataacc gaaagctgcg ggtttgtgtt gctacttgta 121 accgtgcaga ttattctaaa cttgccccga tcatgtttgg cattaaaacc gaacctgagt 181 tctttgaact tgatgttgtg gtacttggct ctcacctgat agatgactat ggaaatacat 241 atcgaatgat tgaacaagat gactttgaca ttaacaccag gctacacaca attgtgaggg 301 gagaagatga ggcagccatg gtggagtcag taggcctggc cctagtgaag ctgccagatg 361 tccttaatcg cctgaagcct gatatcatga ttgttcatgg agacaggttt gatgccctgg 421 ctctggccac atctgctgcc ttgatgaaca tccgaatcct tcacattgaa ggtggggaag 481 tcagtgggac cattgatgac tctatcagac atgccataac aaaactggct cattatcatg 541 tgtgctgcac ccgcagtgca gagcagcacc tgatatccat gtgtgaggac catgatcgca 601 tccttttggc aggctgccct tcctatgaca aacttctctc agccaagaac aaagactaca 661 tgagcatcat tcgcatgtgg ctaggtgatg atgtaaaatc taaagattac attgttgcac 721 tacagcaccc tgtgaccact gacattaagc attccataaa aatgtttgaa ttaacattgg 781 atgcacttat ctcatttaac aagcggaccc tagtcctgtt tccaaatatt gacgcaggga 841 gcaaagagat ggttcgagtg atgcggaaga agggcattga gcatcatccc aactttcgtg 901 cagttaaaca cgtcccattt gaccagttta tacagttggt tgcccatgct ggctgtatga 961 ttgggaacag cagctgtggg gttcgagaag ttggagcttt tggaacacct gtgatcaacc 1021 tgggaacacg tcagattgga agagaaacag gggagaatgt tcttcatgtc cgggatgctg 1081 acacccaaga caaaatattg caagcactgc accttcagtt tggtaaacag tacccttgtt 1141 caaagatata tggggatgga aatgctgttc caaggatttt gaagtttctc aaatctatcg 1201 atcttcaaga gccactgcaa aagaaattct gctttcctcc tgtgaaggag aatatctctc 1261 aagatattga ccatattctt gaaactctaa gtgccttggc cgttgatctt ggcgggacga 1321 acctccgagt tgcaatagtc agcatgaagg gtgaaatagt taagaagtat actcagttca 1381 atcctaaaac ctatgaagag aggattaatt taatcctaca gatgtgtgtg gaagctgcag 1441 cagaagctgt aaaactgaac tgcagaattt tgggagtagg catttccaca ggtggccgtg 1501 taaatcctcg ggaaggaatt gtgctgcatt caaccaaact gatccaagag tggaactctg 1561 tggaccttag gacccccctt tctgacactt tgcatctccc tgtgtgggta gacaatgatg 1621 gcaactgtgc tgccctggcg gaaaggaaat ttggccaagg aaagggactg gaaaactttg 1681 ttacacttat cacaggcaca ggaatcggtg gtggaattat ccatcagcat gaattgatcc 1741 acggaagctc cttctgtgct gcagaactgg gccaccttgt tgtgtctctg gatgggcctg 1801 attgttcctg tggaagccat gggtgcattg aagcatacgc cTCTGGAATG GCCTTGCAGA 1861 GGGAGGCAAA AAAGCTCCAT GATGAGGACC TGCTCTTGGT GGAAGGGATG TCAGTGCCAA 1921 AAGATGAGGC TGTGGGTGCG CTCCATCTCA TCCAAGCTGC GAAACTTGGC AATGCGAAGG 1981 CCCAGAGCAT CCTAAGAACA GCTGGAACAG CTTTGGGTCT TGGGGTTGTG AACATCCTCC 2041 ATACCATGAA TCCCTCCCTT GTGATCCTCT CCGGAGTCCT GGCCAGTCAC TATATCCACA 2101 TTGTCAAAGA CGTCATTCGC CAGCAGGCCT TGTCCTCCGT GCAGGACGTG GATGTGGTGG 2161 TTTCGGATTT GGTTGACCCC GCCCTGCTGG GTGCTGCCAG CATGGTTCTG GACTACACAA 2221 CACGCAGGAT CTACTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2281 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2341 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGGAACACCA CAAGTGTTGC 2401 TTTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt