Construct: ORF TRCN0000478838
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017074.1_s317c1
- Derived from:
- ccsbBroadEn_11846
- DNA Barcode:
- GGCCGAAATTAGCAACATAGGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYTH4 (27128)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478838
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27128 | CYTH4 | cytohesin 4 | NM_013385.5 | 29.9% | 29.9% | 355_1182del |
| 2 | human | 27128 | CYTH4 | cytohesin 4 | NM_001318024.2 | 15.4% | 15.4% | 0_1ins171;184_1011del |
| 3 | mouse | 72318 | Cyth4 | cytohesin 4 | NM_028195.3 | 25.3% | 26.4% | (many diffs) |
| 4 | mouse | 72318 | Cyth4 | cytohesin 4 | XM_006521446.3 | 24.7% | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 420
- ORF length:
- 354
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccTGTGCCAC CCAGAGCCCG CGGAGCTGAG CAGCGGGGAG ACGGAAGAGT 121 TACAGAGGAT CAAGTGGCAC CGAAAGCAGC TCCTGGAGGA CATCCAGAAG CTGAAGGATG 181 AGATTGCAGA TGTGTTTGCC CAAATCGACT GCTTCGAGAG TGCGGAGGAG AGCCGGATGG 241 CCCAGAAGGA GAAGGAGCTG TGTATTGGGC GCAAGAAGTT CAACATGGAC CCCGCCAAGG 301 GTATCCAGTA TTTCATTGAG CACAAGCTGC TGACCCCTGA CGTCCAGGAC ATTGCACGGT 361 TCCTGTATAA AGGCGAGGGC CTCAACAAGA CAGCCATTGG TACCTACCTG GGGGAGAGGT 421 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 481 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 541 TTTATATATC TTGTGGAAAG GACGAGGCCG AAATTAGCAA CATAGGGCTA CGCGTTAAGT 601 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt