Construct: ORF TRCN0000478840
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011815.2_s317c1
- Derived from:
- ccsbBroadEn_02230
- DNA Barcode:
- CCTGATACCTTCCGGGCTCATCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HERPUD1 (9709)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478840
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9709 | HERPUD1 | homocysteine inducible ER p... | NM_014685.4 | 100% | 100% | |
| 2 | human | 9709 | HERPUD1 | homocysteine inducible ER p... | NM_001010989.3 | 99.7% | 99.7% | 223_224insAGC |
| 3 | human | 9709 | HERPUD1 | homocysteine inducible ER p... | NM_001272103.2 | 70.3% | 64.8% | 748_749ins157;825_826ins191 |
| 4 | mouse | 64209 | Herpud1 | homocysteine-inducible, end... | NM_022331.1 | 86.5% | 88.7% | (many diffs) |
| 5 | mouse | 64209 | Herpud1 | homocysteine-inducible, end... | XM_006531260.3 | 69% | 70.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1239
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtccgagacc gaacccgagc ccgtcacgct cctggtgaag agccccaacc 121 agcgccaccg cgacttggag ctgagtggcg accgcggctg gagtgtgggc cacctcaagg 181 cccacctgag ccgcgtctac cccgagcgtc cgcgtccaga ggaccagagg ttaatttatt 241 ctgggaagct gttgttggat caccaatgtc tcagggactt gcttccaaag caggaaaaac 301 ggcatgtttt gcatctggtg tgcaatgtga agagtccttc aaaaatgcca gaaatcaacg 361 ccaaggtggc tgaatccaca gaggagcctg ctggttctaa tcggggacag tatcctgagg 421 attcctcaag tgatggttta aggcaaaggg aagttcttcg gaacctttct tcccctggat 481 gggaaaacat ctcaaggcct gaagctgccc agcaggcatt ccaaggcctg ggtcctggtt 541 tctccggtta cacaccctat gggtggcttc agctttcctg gttccagcag atatatgcac 601 gacagtacta catgcaatat ttagcagcca ctgctgcatc aggggctttt gttccaccac 661 caagtgcaca agagatacct gtggtctctg cacctgctcc agcccctatt cacaaccagt 721 ttccagctga aaaccagcct gccaatcaga atgctgctcc tcaagtggtt gttaatcctG 781 GAGCCAATCA AAATTTGCGG ATGAATGCAC AAGGTGGCCC TATTGTGGAA GAAGATGATG 841 AAATAAATCG AGATTGGTTG GATTGGACCT ATTCAGCAGC TACATTTTCT GTTTTTCTCA 901 GTATCCTCTA CTTCTACTCC TCCCTGAGCA GATTCCTCAT GGTCATGGGG GCCACCGTTG 961 TTATGTACCT GCATCACGTT GGGTGGTTTC CATTTAGACC GAGGCCGGTT CAGAACTTCC 1021 CAAATGATGG TCCTCCTCCT GACGTTGTAA ATCAGGACCC CAACAATAAC TTACAGGAAG 1081 GCACTGATCC TGAAACTGAA GACCCCAACC ACCTCCCTCC AGACAGGGAT GTACTAGATG 1141 GCGAGCAGAC CAGCCCCTCC TTTATGAGCA CAGCATGGCT TGTCTTCAAG ACTTTCTTTG 1201 CCTCTCTTCT TCCAGAAGGC CCCCCAGCCA TCGCAAACTG CCCAACTTTC TTGTACAAAG 1261 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1321 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1381 ACGACCTGAT ACCTTCCGGG CTCATCGCAC GCGTTAAGTC gacaatcaac ctctggatta 1441 caaaatttgt gaaagatt