Construct: ORF TRCN0000478840

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011815.2_s317c1
Derived from:
ccsbBroadEn_02230
DNA Barcode:
CCTGATACCTTCCGGGCTCATCGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HERPUD1 (9709)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478840
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9709 HERPUD1 homocysteine inducible ER p... NM_014685.4 100% 100%
2 human 9709 HERPUD1 homocysteine inducible ER p... NM_001010989.3 99.7% 99.7% 223_224insAGC
3 human 9709 HERPUD1 homocysteine inducible ER p... NM_001272103.2 70.3% 64.8% 748_749ins157;825_826ins191
4 mouse 64209 Herpud1 homocysteine-inducible, end... NM_022331.1 86.5% 88.7% (many diffs)
5 mouse 64209 Herpud1 homocysteine-inducible, end... XM_006531260.3 69% 70.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1239
ORF length:
1173
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gtccgagacc gaacccgagc ccgtcacgct cctggtgaag agccccaacc
121agcgccaccg cgacttggag ctgagtggcg accgcggctg gagtgtgggc cacctcaagg
181cccacctgag ccgcgtctac cccgagcgtc cgcgtccaga ggaccagagg ttaatttatt
241ctgggaagct gttgttggat caccaatgtc tcagggactt gcttccaaag caggaaaaac
301ggcatgtttt gcatctggtg tgcaatgtga agagtccttc aaaaatgcca gaaatcaacg
361ccaaggtggc tgaatccaca gaggagcctg ctggttctaa tcggggacag tatcctgagg
421attcctcaag tgatggttta aggcaaaggg aagttcttcg gaacctttct tcccctggat
481gggaaaacat ctcaaggcct gaagctgccc agcaggcatt ccaaggcctg ggtcctggtt
541tctccggtta cacaccctat gggtggcttc agctttcctg gttccagcag atatatgcac
601gacagtacta catgcaatat ttagcagcca ctgctgcatc aggggctttt gttccaccac
661caagtgcaca agagatacct gtggtctctg cacctgctcc agcccctatt cacaaccagt
721ttccagctga aaaccagcct gccaatcaga atgctgctcc tcaagtggtt gttaatcctG
781GAGCCAATCA AAATTTGCGG ATGAATGCAC AAGGTGGCCC TATTGTGGAA GAAGATGATG
841AAATAAATCG AGATTGGTTG GATTGGACCT ATTCAGCAGC TACATTTTCT GTTTTTCTCA
901GTATCCTCTA CTTCTACTCC TCCCTGAGCA GATTCCTCAT GGTCATGGGG GCCACCGTTG
961TTATGTACCT GCATCACGTT GGGTGGTTTC CATTTAGACC GAGGCCGGTT CAGAACTTCC
1021CAAATGATGG TCCTCCTCCT GACGTTGTAA ATCAGGACCC CAACAATAAC TTACAGGAAG
1081GCACTGATCC TGAAACTGAA GACCCCAACC ACCTCCCTCC AGACAGGGAT GTACTAGATG
1141GCGAGCAGAC CAGCCCCTCC TTTATGAGCA CAGCATGGCT TGTCTTCAAG ACTTTCTTTG
1201CCTCTCTTCT TCCAGAAGGC CCCCCAGCCA TCGCAAACTG CCCAACTTTC TTGTACAAAG
1261TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1321GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1381ACGACCTGAT ACCTTCCGGG CTCATCGCAC GCGTTAAGTC gacaatcaac ctctggatta
1441caaaatttgt gaaagatt

Download FASTA (ORF) (Full)