Construct: ORF TRCN0000478848
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016835.1_s317c1
- Derived from:
- ccsbBroadEn_06295
- DNA Barcode:
- GGCTACTGCGGCAAGCCGTCTGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNAZ (2781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478848
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2781 | GNAZ | G protein subunit alpha z | NM_002073.4 | 99.8% | 99.7% | 193G>T;687C>T |
| 2 | human | 2781 | GNAZ | G protein subunit alpha z | XM_017028786.2 | 73.7% | 66% | (many diffs) |
| 3 | human | 2781 | GNAZ | G protein subunit alpha z | XR_937843.3 | 29% | (many diffs) | |
| 4 | mouse | 14687 | Gnaz | guanine nucleotide binding ... | NM_010311.3 | 92.2% | 98% | (many diffs) |
| 5 | mouse | 14687 | Gnaz | guanine nucleotide binding ... | XM_006513235.3 | 92.2% | 98% | (many diffs) |
| 6 | mouse | 14687 | Gnaz | guanine nucleotide binding ... | XM_006513236.1 | 92.2% | 98% | (many diffs) |
| 7 | mouse | 14687 | Gnaz | guanine nucleotide binding ... | XM_006513237.2 | 92.2% | 98% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1131
- ORF length:
- 1065
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg atgtcggcaa agctcagagg aaaaagaagc agcccggcgg tcccggagaa 121 ttgaccgcca cctgcgctca gagagccagc ggcaacgccg cgaaatcaag ctgctcctgc 181 tgggcaccag caactcaggc aagagcacca tcgtcaaaca gatgaagatc atccacagcg 241 gcggcttcaa cctggagtcc tgcaaggagt acaagcccct catcatctac aatgccatcg 301 actcgctgac ccgcatcatc cgggccctgg ccgccctcag gatcgacttc cacaaccccg 361 accgcgccta cgacgctgtg cagctctttg cgctgacggg ccccgctgag agcaagggcg 421 agatcacacc cgagctgctg ggtgtcatgc gacggctctg ggccgaccca ggggcacagg 481 cctgcttcag ccgctccagc gagtaccacc tggaggacaa cgcggcctac tacctgaacg 541 acctggagcg catcgccgca gctgactata tccccactgt cgaggacatc ctgcgctccc 601 gggacatgac cacgggcatt gtggagaaca agttcacctt caaggagctc accttcaaga 661 tggtggacgt gggggggcag aggtcagagc gcaaaaagtg gatccactgc ttcgagggcg 721 tcacagccat catcttctgt gtggagctca gtggctacga cctgaaactc tacgaggata 781 accagacaag tcggatggca gagagcttgc gcctctttga ctccatctgc aacaacaact 841 ggttcatcaa cacctcactc aTCCTCTTCC TGAACAAGAA GGACCTGCTG GCAGAGAAGA 901 TCCGCCGCAT CCCGCTCACC ATCTGCTTTC CCGAGTACAA GGGCCAGAAC ACGTACGAGG 961 AGGCCGCTGT CTACATCCAG CGGCAGTTTG AAGACCTGAA CCGCAACAAG GAGACCAAGG 1021 AGATCTACTC CCACTTCACC TGCGCCACCG ACACCAGTAA CATCCAGTTT GTCTTCGACG 1081 CGGTGACAGA CGTCATCATA CAGAACAATC TCAAGTACAT TGGCCTTTGC TGCCCAACTT 1141 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1201 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1261 CTTGTGGAAA GGACGAGGCT ACTGCGGCAA GCCGTCTGGA ACGCGTTAAG TCgacaatca 1321 acctctggat tacaaaattt gtgaaagatt