Construct: ORF TRCN0000478855

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010240.1_s317c1
Derived from:
ccsbBroadEn_10732
DNA Barcode:
TATACTCCTCCAAGGTGGACAACT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHD1 (1105)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478855
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1105 CHD1 chromodomain helicase DNA b... NM_001270.2 99.9% 99.9% 4335G>A;5048_5050delCTC
2 human 1105 CHD1 chromodomain helicase DNA b... XM_005271867.5 99.9% 99.9% 4335G>A;5048_5050delCTC
3 human 1105 CHD1 chromodomain helicase DNA b... NM_001364113.1 95% 95% 4108_4371del;4599G>A;5312_5314delCTC
4 human 1105 CHD1 chromodomain helicase DNA b... XM_024454344.1 95% 95% 4108_4371del;4599G>A;5312_5314delCTC
5 human 1105 CHD1 chromodomain helicase DNA b... XM_024454345.1 86.3% 84.4% (many diffs)
6 human 1105 CHD1 chromodomain helicase DNA b... NR_157078.1 66.9% (many diffs)
7 human 1105 CHD1 chromodomain helicase DNA b... NR_157079.1 64.8% (many diffs)
8 human 1105 CHD1 chromodomain helicase DNA b... XR_001741986.2 64.5% (many diffs)
9 human 1105 CHD1 chromodomain helicase DNA b... XR_002959841.1 62.1% (many diffs)
10 human 1105 CHD1 chromodomain helicase DNA b... XM_017008991.1 43.3% 43.3% (many diffs)
11 human 1105 CHD1 chromodomain helicase DNA b... XM_017008992.1 43.3% 43.3% (many diffs)
12 mouse 12648 Chd1 chromodomain helicase DNA b... NM_007690.3 87.8% 95.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
5196
ORF length:
5127
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaatggacac agtgatgaag aaagtgttag aaacagtagt ggagaatcaa
121gccagtcgga tgatgattct gggtcagctt caggctctgg atctggttcg agttctggaa
181gcagtagtga tggaagcagt agccagtcag gtagcagtga ctctgactcc ggatctgaat
241caggcagtca gtcagagtct gagtcagaca cttcccgaga aaacaaagtt caagcaaaac
301caccgaaagt tgatggagct gagttttgga aatctagtcc tagtattctg gccgttcaga
361gatctgcaat cctcaagaag cagcaacagc agcagcagca acaacaacat caagcctcat
421ctaatagcgg atcagaagag gattcctcta gcagtgaaga ttccgatgac tcatcaagtg
481aggtcaaaag gaaaaagcat aaagatgaag attggcaaat gtctgggtca ggatctccat
541ctcagtctgg ttcagattca gaatctgaag aagagagaga gaaaagcagt tgtgatgaaa
601cagaatctga ttatgagcca aaaaacaaag tcaaaagcag aaaacctcaa aatagatcta
661agtcaaaaaa tggaaagaag attcttggac aaaaaaagag acagattgat tcatctgagg
721aggatgatga tgaagaagat tatgataatg ataaaagaag ttctcgtcgc caagcaactg
781ttaatgttag ctataaggag gatgaagaaa tgaaaacaga ttctgatgac ctactggaag
841tctgtggaga ggatgttcct caacctgagg aagaggaatt tgaaaccata gaaagattta
901tggattgtcg gattgggaga aaaggagcta ctggtgctac tacaaccatc tatgcagttg
961aagcagatgg tgacccaaat gcaggctttg aaaaaaacaa agaaccagga gagattcagt
1021atttaattaa atggaaagga tggtcccata tccacaacac ttgggagaca gaagaaaccc
1081tcaagcagca gaatgttaga ggaatgaaaa aattggataa ttataagaaa aaagatcagg
1141aaacaaaaag atggttgaaa aatgcctctc cagaagatgt ggaatattat aattgccagc
1201aagaacttac agatgatcta cataaacagt atcaaatagt ggaacgtata attgctcatt
1261ccaatcaaaa gtcagcagct ggttatcctg attattactg caaatggcag ggccttccat
1321actcagagtg cagctgggaa gatggagctc tcatttccaa aaagtttcaa gcatgcattg
1381atgagtattt tagcaggaac caatcaaaaa ccactccttt taaagattgc aaagtattaa
1441aacaaaggcc aaggtttgta gccctgaaga agcagccatc ctatattgga ggacatgagg
1501gcttagaatt aagagattat caactgaatg gtttaaattg gcttgctcat tcttggtgca
1561aaggaaatag ttgcatactc gctgatgaaa tgggccttgg aaaaacaata cagacgatct
1621catttctgaa ttatttgttt catgaacatc aattatatgg acctttttta ttggtagtac
1681cgctctccac tcttacttcc tggcaaaggg aaattcagac ttgggcttct caaatgaatg
1741ctgtggttta tttaggtgac attaacagca gaaacatgat aagaactcat gaatggacgc
1801atcatcagac caaacggtta aaatttaata tattgttaac aacttatgaa attttattaa
1861aagataaggc attccttgga ggtctaaatt gggcatttat aggtgttgat gaagcacacc
1921gattaaagaa tgatgactcc cttctgtata aaactttaat agattttaaa tccaatcatc
1981gtctccttat cactggaact cctctacaga attccctcaa agagctctgg tctttgctac
2041atttcattat gccagaaaag ttttcttcct gggaagattt tgaagaagaa catggcaaag
2101ggagagaata tggttatgca agccttcaca aggagcttga gccatttctg ttacgccgag
2161ttaagaaaga tgtggaaaaa tctcttcctg ccaaggttga gcagatttta agaatggaaa
2221tgagtgcttt acagaaacaa tattacaaat ggattttaac taggaattac aaagccctca
2281gcaaaggttc caagggcagt acctcaggct ttttgaacat tatgatggag ctaaagaaat
2341gttgtaacca ttgctacctc attaaaccac cagataataa tgaattctat aataaacagg
2401aggccttaca acacttaatt cgtagtagcg gaaaattgat tcttcttgac aagctattaa
2461ttcgcctaag agaacgaggc aatcgagttc ttattttttc acaaatggtg cggatgttag
2521atatacttgc agaatatttg aaatatcgtc aattcccctt tcaaagatta gatggatcaa
2581taaaaggaga actgaggaaa caagctctag atcattttaa tgctgaggga tcagaggatt
2641tttgcttttt gctgtccaca agagctggag gtctagggat taatttagcc tctgctgaca
2701ctgttgttat atttgattcc gattggaatc cacagaatga tcttcaggca caggctagag
2761cccatcgaat tgggcaaaag aaacaggtga atatttatcg tctagttaca aagggatcag
2821ttgaagaaga tattcttgaa agggcgaaaa agaagatggt tttagatcat cttgtaattc
2881aaagaatgga cacaactggg aagacagtac tacatacagg ttctgcccca tcaagttcta
2941ctcctttcaa taaagaagag ttatcagcca ttttaaagtt tggtgctgaa gaacttttta
3001aggaacctga aggagaagaa caagagcccc aggaaatgga tatagatgaa atcttgaaga
3061gagctgaaac tcatgaaaat gaaccaggtc ctttaactgt aggagatgaa ttgctttccc
3121agttcaaggt tgccaacttc tcaaatatgg atgaggatga cattgagttg gaacctgaaa
3181gaaattcaaa gaattgggag gaaattattc cagaagatca aagaagacga ttagaagaag
3241aagaaagaca aaaggaactt gaagaaattt atatgctccc aagaatgaga aattgtgcaa
3301aacagattag tttcaatgga agtgaaggga ggcgcagtag aagtaggaga tactctggat
3361ctgatagtga ttccatctca gaagggaaaa ggccaaagaa acgtggaaga ccacggacta
3421ttcctcggga gaatattaaa ggatttagtg atgcagaaat taggcggttt atcaagagct
3481ataagaaatt tggtggtcct ctggaaagat tagatgcaat tgctcgagat gctgagttag
3541ttgataagtc agaaacagac cttagacgac tgggagaatt ggtacataat ggttgcatta
3601aagcattaaa ggatagttct tcaggaacag aacgaacagg tggtagactc ggaaaagtga
3661agggtccaac attccgaata tcaggagtac aggtgaatgc caaactagtc atctcccatg
3721aagaagaatt aatacctttg cacaaatcca ttccttctga tccagaagaa agaaagcagt
3781atactatccc atgccacaca aaggcagctc attttgatat agactggggc aaagaagatg
3841attccaattt gttaattggc atctatgaat atggatatgg aagctgggaa atgattaaaa
3901tggatcctga cctcagtcta acacacaaga ttcttccaga tgatcccgat aaaaaaccac
3961aagcaaaaca gttgcagacc cgtgcagact acctcatcaa attacttagt agagatcttg
4021caaaaaaaga agctctttct ggtgcgggaa gttcaaagag gagaaaagca agagctaaga
4081agaataaagc aatgaagtct ataaaagtga aagaggaaat aaagagtgat tcttctcctc
4141tgccttcaga gaagtctgat gaagatgatg ataaattgag tgaatccaag tctgatggta
4201gggaaagatc caagaaatct tcagtgtcag atgctccagt tcatatcacg gcaagtggtg
4261aaccagttcc catttctgaa gaatctgaag agctggatca gaagacattc agcatttgta
4321aagaaagaat gaggcctgtt aaagcagctt tgaaacaact tgataggcct gagaaaggcc
4381tttcagaaag agaacaacta gaacatacta gacaatgttt aataaaaatt ggagaccata
4441tcacagaatg tctaaaagag tatacaaatc ctgaacaaat taagcaatgg agaaaaaacc
4501tgtggatttt tgtatctaag tttactgaat ttgatgcaag aaaattacat aaattatata
4561agcatgctat taaaaaacgg caggagtctc agcaaaacag tgatcaaaac agcaacttga
4621atcctcacgt gattagaaat ccagatgtgg aaagattaaa agagaataca aatcacgatg
4681atagcagcag ggacagttat tcctctgata gacacttaac tcagtaccat gatcatcata
4741aagaccgaca tcagggagat tcttacaaaa aaagtgattc caggaaaaga ccctattctt
4801cttttagtaa tggtaaagac catcgtgatt GGGATCACTA CAAGCAAGAC AGCAGATATT
4861ACAGTGACAG AGAGAAACAC AGAAAACTGG ATGATCACAG GAGTAGAGAT CACAGGTCAA
4921ATTTGGAAGG AAGTTTAAAA GATAGATCTC ATTCTGATCA TCGTTCTCAC TCAGATCATC
4981GGTTACATTC AGACCACCGG TCAAGTTCTG AATATACGCA CCATAAATCT TCCAGGGATT
5041ATAGGTATCA CTCAGACTGG CAAATGGACC ACAGAGCTTC CAGCAGTGGC CCTAGGTCAC
5101CACTAGATCA GAGATCTTAT GGCTCCAGAT CTCCATTTGA ACATTCAGTT GAACACAAAA
5161GTACACCGGA GCATACCTGG AGTAGTCGGA AAACATTGCC AACTTTCTTG TACAAAGTGG
5221TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
5281CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
5341ATATACTCCT CCAAGGTGGA CAACTACGCG TTAAGTCgac aatcaacctc tggattacaa
5401aatttgtgaa agatt

Download FASTA (ORF) (Full)

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