Construct: ORF TRCN0000478855
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010240.1_s317c1
- Derived from:
- ccsbBroadEn_10732
- DNA Barcode:
- TATACTCCTCCAAGGTGGACAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHD1 (1105)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478855
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1105 | CHD1 | chromodomain helicase DNA b... | NM_001270.2 | 99.9% | 99.9% | 4335G>A;5048_5050delCTC |
| 2 | human | 1105 | CHD1 | chromodomain helicase DNA b... | XM_005271867.5 | 99.9% | 99.9% | 4335G>A;5048_5050delCTC |
| 3 | human | 1105 | CHD1 | chromodomain helicase DNA b... | NM_001364113.1 | 95% | 95% | 4108_4371del;4599G>A;5312_5314delCTC |
| 4 | human | 1105 | CHD1 | chromodomain helicase DNA b... | XM_024454344.1 | 95% | 95% | 4108_4371del;4599G>A;5312_5314delCTC |
| 5 | human | 1105 | CHD1 | chromodomain helicase DNA b... | XM_024454345.1 | 86.3% | 84.4% | (many diffs) |
| 6 | human | 1105 | CHD1 | chromodomain helicase DNA b... | NR_157078.1 | 66.9% | (many diffs) | |
| 7 | human | 1105 | CHD1 | chromodomain helicase DNA b... | NR_157079.1 | 64.8% | (many diffs) | |
| 8 | human | 1105 | CHD1 | chromodomain helicase DNA b... | XR_001741986.2 | 64.5% | (many diffs) | |
| 9 | human | 1105 | CHD1 | chromodomain helicase DNA b... | XR_002959841.1 | 62.1% | (many diffs) | |
| 10 | human | 1105 | CHD1 | chromodomain helicase DNA b... | XM_017008991.1 | 43.3% | 43.3% | (many diffs) |
| 11 | human | 1105 | CHD1 | chromodomain helicase DNA b... | XM_017008992.1 | 43.3% | 43.3% | (many diffs) |
| 12 | mouse | 12648 | Chd1 | chromodomain helicase DNA b... | NM_007690.3 | 87.8% | 95.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5196
- ORF length:
- 5127
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaatggacac agtgatgaag aaagtgttag aaacagtagt ggagaatcaa 121 gccagtcgga tgatgattct gggtcagctt caggctctgg atctggttcg agttctggaa 181 gcagtagtga tggaagcagt agccagtcag gtagcagtga ctctgactcc ggatctgaat 241 caggcagtca gtcagagtct gagtcagaca cttcccgaga aaacaaagtt caagcaaaac 301 caccgaaagt tgatggagct gagttttgga aatctagtcc tagtattctg gccgttcaga 361 gatctgcaat cctcaagaag cagcaacagc agcagcagca acaacaacat caagcctcat 421 ctaatagcgg atcagaagag gattcctcta gcagtgaaga ttccgatgac tcatcaagtg 481 aggtcaaaag gaaaaagcat aaagatgaag attggcaaat gtctgggtca ggatctccat 541 ctcagtctgg ttcagattca gaatctgaag aagagagaga gaaaagcagt tgtgatgaaa 601 cagaatctga ttatgagcca aaaaacaaag tcaaaagcag aaaacctcaa aatagatcta 661 agtcaaaaaa tggaaagaag attcttggac aaaaaaagag acagattgat tcatctgagg 721 aggatgatga tgaagaagat tatgataatg ataaaagaag ttctcgtcgc caagcaactg 781 ttaatgttag ctataaggag gatgaagaaa tgaaaacaga ttctgatgac ctactggaag 841 tctgtggaga ggatgttcct caacctgagg aagaggaatt tgaaaccata gaaagattta 901 tggattgtcg gattgggaga aaaggagcta ctggtgctac tacaaccatc tatgcagttg 961 aagcagatgg tgacccaaat gcaggctttg aaaaaaacaa agaaccagga gagattcagt 1021 atttaattaa atggaaagga tggtcccata tccacaacac ttgggagaca gaagaaaccc 1081 tcaagcagca gaatgttaga ggaatgaaaa aattggataa ttataagaaa aaagatcagg 1141 aaacaaaaag atggttgaaa aatgcctctc cagaagatgt ggaatattat aattgccagc 1201 aagaacttac agatgatcta cataaacagt atcaaatagt ggaacgtata attgctcatt 1261 ccaatcaaaa gtcagcagct ggttatcctg attattactg caaatggcag ggccttccat 1321 actcagagtg cagctgggaa gatggagctc tcatttccaa aaagtttcaa gcatgcattg 1381 atgagtattt tagcaggaac caatcaaaaa ccactccttt taaagattgc aaagtattaa 1441 aacaaaggcc aaggtttgta gccctgaaga agcagccatc ctatattgga ggacatgagg 1501 gcttagaatt aagagattat caactgaatg gtttaaattg gcttgctcat tcttggtgca 1561 aaggaaatag ttgcatactc gctgatgaaa tgggccttgg aaaaacaata cagacgatct 1621 catttctgaa ttatttgttt catgaacatc aattatatgg acctttttta ttggtagtac 1681 cgctctccac tcttacttcc tggcaaaggg aaattcagac ttgggcttct caaatgaatg 1741 ctgtggttta tttaggtgac attaacagca gaaacatgat aagaactcat gaatggacgc 1801 atcatcagac caaacggtta aaatttaata tattgttaac aacttatgaa attttattaa 1861 aagataaggc attccttgga ggtctaaatt gggcatttat aggtgttgat gaagcacacc 1921 gattaaagaa tgatgactcc cttctgtata aaactttaat agattttaaa tccaatcatc 1981 gtctccttat cactggaact cctctacaga attccctcaa agagctctgg tctttgctac 2041 atttcattat gccagaaaag ttttcttcct gggaagattt tgaagaagaa catggcaaag 2101 ggagagaata tggttatgca agccttcaca aggagcttga gccatttctg ttacgccgag 2161 ttaagaaaga tgtggaaaaa tctcttcctg ccaaggttga gcagatttta agaatggaaa 2221 tgagtgcttt acagaaacaa tattacaaat ggattttaac taggaattac aaagccctca 2281 gcaaaggttc caagggcagt acctcaggct ttttgaacat tatgatggag ctaaagaaat 2341 gttgtaacca ttgctacctc attaaaccac cagataataa tgaattctat aataaacagg 2401 aggccttaca acacttaatt cgtagtagcg gaaaattgat tcttcttgac aagctattaa 2461 ttcgcctaag agaacgaggc aatcgagttc ttattttttc acaaatggtg cggatgttag 2521 atatacttgc agaatatttg aaatatcgtc aattcccctt tcaaagatta gatggatcaa 2581 taaaaggaga actgaggaaa caagctctag atcattttaa tgctgaggga tcagaggatt 2641 tttgcttttt gctgtccaca agagctggag gtctagggat taatttagcc tctgctgaca 2701 ctgttgttat atttgattcc gattggaatc cacagaatga tcttcaggca caggctagag 2761 cccatcgaat tgggcaaaag aaacaggtga atatttatcg tctagttaca aagggatcag 2821 ttgaagaaga tattcttgaa agggcgaaaa agaagatggt tttagatcat cttgtaattc 2881 aaagaatgga cacaactggg aagacagtac tacatacagg ttctgcccca tcaagttcta 2941 ctcctttcaa taaagaagag ttatcagcca ttttaaagtt tggtgctgaa gaacttttta 3001 aggaacctga aggagaagaa caagagcccc aggaaatgga tatagatgaa atcttgaaga 3061 gagctgaaac tcatgaaaat gaaccaggtc ctttaactgt aggagatgaa ttgctttccc 3121 agttcaaggt tgccaacttc tcaaatatgg atgaggatga cattgagttg gaacctgaaa 3181 gaaattcaaa gaattgggag gaaattattc cagaagatca aagaagacga ttagaagaag 3241 aagaaagaca aaaggaactt gaagaaattt atatgctccc aagaatgaga aattgtgcaa 3301 aacagattag tttcaatgga agtgaaggga ggcgcagtag aagtaggaga tactctggat 3361 ctgatagtga ttccatctca gaagggaaaa ggccaaagaa acgtggaaga ccacggacta 3421 ttcctcggga gaatattaaa ggatttagtg atgcagaaat taggcggttt atcaagagct 3481 ataagaaatt tggtggtcct ctggaaagat tagatgcaat tgctcgagat gctgagttag 3541 ttgataagtc agaaacagac cttagacgac tgggagaatt ggtacataat ggttgcatta 3601 aagcattaaa ggatagttct tcaggaacag aacgaacagg tggtagactc ggaaaagtga 3661 agggtccaac attccgaata tcaggagtac aggtgaatgc caaactagtc atctcccatg 3721 aagaagaatt aatacctttg cacaaatcca ttccttctga tccagaagaa agaaagcagt 3781 atactatccc atgccacaca aaggcagctc attttgatat agactggggc aaagaagatg 3841 attccaattt gttaattggc atctatgaat atggatatgg aagctgggaa atgattaaaa 3901 tggatcctga cctcagtcta acacacaaga ttcttccaga tgatcccgat aaaaaaccac 3961 aagcaaaaca gttgcagacc cgtgcagact acctcatcaa attacttagt agagatcttg 4021 caaaaaaaga agctctttct ggtgcgggaa gttcaaagag gagaaaagca agagctaaga 4081 agaataaagc aatgaagtct ataaaagtga aagaggaaat aaagagtgat tcttctcctc 4141 tgccttcaga gaagtctgat gaagatgatg ataaattgag tgaatccaag tctgatggta 4201 gggaaagatc caagaaatct tcagtgtcag atgctccagt tcatatcacg gcaagtggtg 4261 aaccagttcc catttctgaa gaatctgaag agctggatca gaagacattc agcatttgta 4321 aagaaagaat gaggcctgtt aaagcagctt tgaaacaact tgataggcct gagaaaggcc 4381 tttcagaaag agaacaacta gaacatacta gacaatgttt aataaaaatt ggagaccata 4441 tcacagaatg tctaaaagag tatacaaatc ctgaacaaat taagcaatgg agaaaaaacc 4501 tgtggatttt tgtatctaag tttactgaat ttgatgcaag aaaattacat aaattatata 4561 agcatgctat taaaaaacgg caggagtctc agcaaaacag tgatcaaaac agcaacttga 4621 atcctcacgt gattagaaat ccagatgtgg aaagattaaa agagaataca aatcacgatg 4681 atagcagcag ggacagttat tcctctgata gacacttaac tcagtaccat gatcatcata 4741 aagaccgaca tcagggagat tcttacaaaa aaagtgattc caggaaaaga ccctattctt 4801 cttttagtaa tggtaaagac catcgtgatt GGGATCACTA CAAGCAAGAC AGCAGATATT 4861 ACAGTGACAG AGAGAAACAC AGAAAACTGG ATGATCACAG GAGTAGAGAT CACAGGTCAA 4921 ATTTGGAAGG AAGTTTAAAA GATAGATCTC ATTCTGATCA TCGTTCTCAC TCAGATCATC 4981 GGTTACATTC AGACCACCGG TCAAGTTCTG AATATACGCA CCATAAATCT TCCAGGGATT 5041 ATAGGTATCA CTCAGACTGG CAAATGGACC ACAGAGCTTC CAGCAGTGGC CCTAGGTCAC 5101 CACTAGATCA GAGATCTTAT GGCTCCAGAT CTCCATTTGA ACATTCAGTT GAACACAAAA 5161 GTACACCGGA GCATACCTGG AGTAGTCGGA AAACATTGCC AACTTTCTTG TACAAAGTGG 5221 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 5281 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 5341 ATATACTCCT CCAAGGTGGA CAACTACGCG TTAAGTCgac aatcaacctc tggattacaa 5401 aatttgtgaa agatt