Construct: ORF TRCN0000478856
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009115.1_s317c1
- Derived from:
- ccsbBroadEn_01700
- DNA Barcode:
- CCAACAACGACGCTCCTGAGAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPH1 (7166)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478856
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7166 | TPH1 | tryptophan hydroxylase 1 | NM_004179.3 | 100% | 100% | |
| 2 | mouse | 21990 | Tph1 | tryptophan hydroxylase 1 | NM_001136084.2 | 85.3% | 89.4% | (many diffs) |
| 3 | mouse | 21990 | Tph1 | tryptophan hydroxylase 1 | NM_009414.3 | 85.3% | 89.4% | (many diffs) |
| 4 | mouse | 21990 | Tph1 | tryptophan hydroxylase 1 | XM_011250846.2 | 67.3% | 71.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1401
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gattgaagac aataaggaga acaaagacca ttccttagaa aggggaagag 121 caagtctcat tttttcctta aagaatgaag ttggaggact tataaaagcc ctgaaaatct 181 ttcaggagaa gcatgtgaat ctgttacata tcgagtcccg aaaatcaaaa agaagaaact 241 cagaatttga gatttttgtt gactgtgaca tcaacagaga acaattgaat gatatttttc 301 atctgctgaa gtctcatacc aatgttctct ctgtgaatct accagataat tttactttga 361 aggaagatgg tatggaaact gttccttggt ttccaaagaa gatttctgac ctggaccatt 421 gtgccaacag agttctgatg tatggatctg aactagatgc agaccatcct ggcttcaaag 481 acaatgtcta ccgtaaacgt cgaaagtatt ttgcggactt ggctatgaac tataaacatg 541 gagaccccat tccaaaggtt gaattcactg aagaggagat taagacctgg ggaaccgtat 601 tccaagagct caacaaactc tacccaaccc atgcttgcag agagtatctc aaaaacttac 661 ctttgctttc taaatattgt ggatatcggg aggataatat cccacaattg gaagatgtct 721 ccaacttttt aaaagagcgt acaggttttt ccatccgtcc tgtggctggt tacttatcac 781 caagagattt cttatcaggt ttagcctttc gagtttttca ctgcactcaa tatgtgagac 841 acagttcaga tcccttctat accccagagc cagatacctg ccatgaactc ttaggtcatg 901 tcccgctttt ggctgaacct agttttgccc aattctccca agaaattggc ttggcttctc 961 ttggcgcttc agaggaggct gttcaaaaac tggcaacgtg ctactttttc actgtggagt 1021 ttggtctatg taaacaagat ggacagcTAA GAGTCTTTGG TGCTGGCTTA CTTTCTTCTA 1081 TCAGTGAACT CAAACATGCA CTTTCTGGAC ATGCCAAAGT AAAGCCCTTT GATCCCAAGA 1141 TTACCTGCAA ACAGGAATGT CTTATCACAA CTTTTCAAGA TGTCTACTTT GTATCTGAAA 1201 GTTTTGAAGA TGCAAAGGAG AAGATGAGAG AATTTACCAA AACAATTAAG CGTCCATTTG 1261 GAGTGAAGTA TAATCCATAT ACACGGAGTA TTCAGATCCT GAAAGACACC AAGAGCATAA 1321 CCAGTGCCAT GAATGAGCTG CAGCATGATC TCGATGTTGT CAGTGATGCC CTTGCTAAGG 1381 TCAGCAGGAA GCCGAGTATC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1441 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1501 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCAA CAACGACGCT 1561 CCTGAGAGAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt