Construct: ORF TRCN0000478860
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001249.2_s317c1
- Derived from:
- ccsbBroadEn_09932
- DNA Barcode:
- GTCAGTGCAGGGATTAACGTATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C10orf53 (282966)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478860
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 282966 | C10orf53 | chromosome 10 open reading ... | NM_182554.4 | 99.3% | 98.7% | 216C>T;295A>G;353C>T |
| 2 | human | 282966 | C10orf53 | chromosome 10 open reading ... | NM_001042427.3 | 52.5% | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caagaacgca gtggtcatcc tgcgctatgg gccctacagc gcggcaggcc 121 taccggtgga gcaccacacc ttccgcctgc agggcctgca agctgtgttG GCCATAGATG 181 GACATGAGGT CATCCTAGAG AAGATAGAAG ACTGGAATGT GGTGGAACTC ATGGTGAATG 241 AAGAAGTCAT CTTCCACTGC AACATTAAGG ACTTGGAGTT TGGCAAGCTC ACCCCAAGTA 301 GTGACAAGAG GACCACCAGC AGCTCCAGGC TGACATTTCA CCAGCTTAGC AGCCCGTGTG 361 GGATGAAAGT TTCTCCTTTG CAACAGTTCC CACAAAAGAC CCAGGACCTG ACTTGTATTG 421 TATTGGCCCA GATTGGATCA TGTATCCATT TCCAAACCAA TCTTTGTGAC CTGGGTTGGC 481 CAGGCCTGGA TCACATGCTC ATTTCTGGAC TAGAGAAGAG GGGAACTCAG CCCTACTGCC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GAGTCAGTGC AGGGATTAAC GTATAAACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt