Construct: ORF TRCN0000478865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017282.2_s317c1
- Derived from:
- ccsbBroadEn_08298
- DNA Barcode:
- ACTGTGATCAAAACATTAACCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTCB (51493)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478865
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51493 | RTCB | RNA 2',3'-cyclic phosphate ... | NM_014306.5 | 99.8% | 99.8% | 4A>C;603G>A |
| 2 | mouse | 28088 | Rtcb | RNA 2',3'-cyclic phosphate ... | NM_145422.4 | 91.8% | 99.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1581
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg tcgcagctat aatgatgagc tgcagttctt ggagaagatc aataaaaact 121 gctggaggat caagaagggc ttcgtgccca acatgcaggt tgaaggtgtt ttctatgtga 181 atgatgctct ggagaaattg atgtttgagg aattaaggaa tgcctgtcga ggtggtggtg 241 ttggtggctt cctgccagcc atgaaacaga ttggcaatgt ggcagccctg cctggaattg 301 ttcatcgatc tattgggctt cctgatgtcc attcaggata tgggtttgct attgggaaca 361 tggcagcctt tgatatgaat gaccctgaag cagtagtatc cccaggtggt gtcgggtttg 421 acatcaactg tggtgtccgc ttgctaagaa ccaatttaga tgaaagtgat gtccagcctg 481 tgaaggagca acttgcccaa gctatgtttg accacattcc tgttggggtg gggtcaaaag 541 gtgtcatccc aatgaatgcc aaagacttgg aggaggcctt ggagatgggg gtggactggt 601 ccttaagaga agggtatgcc tgggctgaag acaaggagca ctgcgaggag tacggaagga 661 tgctgcaagc tgaccccaat aaagtttctg caagggcgaa gaaaagaggc cttcctcagt 721 tggggaccct gggagcaggc aaccattatg cagaaatcca ggttgtggat gagattttca 781 atgagtatgc tgctaaaaaa atgggcatcg accataaggg acaggtgtgt gtgatgatcc 841 acagtggaag cagaggcttg ggccaccaag tagccacaga tgcgctggta gctatggaga 901 aggccatgaa gagagacaag attatagtca atgatcggca gttggcttgt gctcgaatcg 961 cttccccaga gggtcaagac tatctgaagg gaatggcagc tgctgggaac tatgcctggg 1021 tcaaccgctc ttccatgacc ttcttaaccc gtcaggcttt cgccaaggtc ttcaacacaa 1081 cccctgatga cttggaccta catgtgatct atgatgtttc tcacaacatt gccaaagtgg 1141 agcagcatgt ggtggacgga aaggaacgga cactgttagt acacaggaag ggatccaccc 1201 gcgctttccc TCCTCACCAT CCCCTCATTG CTGTTGATTA CCAACTCACT GGACAGCCAG 1261 TGCTCATTGG TGGCACCATG GGAACCTGTA GTTATGTTCT TACTGGCACT GAACAGGGCA 1321 TGACTGAGAC CTTTGGAACA ACCTGTCATG GAGCGGGCCG TGCATTGTCC CGAGCAAAAT 1381 CTCGACGTAA TTTAGATTTC CAGGATGTCT TAGACAAATT GGCAGATATG GGAATTGCGA 1441 TCCGTGTTGC CTCACCCAAA CTGGTTATGG AAGAGGCTCC TGAGTCCTAT AAGAATGTGA 1501 CAGATGTGGT AAATACCTGC CATGATGCTG GAATCAGCAA GAAAGCCATT AAACTGAGAC 1561 CAATTGCTGT GATCAAAGGA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1621 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1681 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACTG TGATCAAAAC 1741 ATTAACCTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt