Construct: ORF TRCN0000478873
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007386.2_s317c1
- Derived from:
- ccsbBroadEn_06427
- DNA Barcode:
- TGCACCCTTGTACCACCCCTATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFIT3 (3437)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478873
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3437 | IFIT3 | interferon induced protein ... | NM_001031683.4 | 99.8% | 99.5% | 83A>T;527T>C |
| 2 | human | 3437 | IFIT3 | interferon induced protein ... | NM_001549.6 | 99.8% | 99.5% | 83A>T;527T>C |
| 3 | human | 3437 | IFIT3 | interferon induced protein ... | NM_001289758.2 | 89.3% | 89.1% | 0_1ins156;371T>C |
| 4 | human | 3437 | IFIT3 | interferon induced protein ... | NM_001289759.2 | 89.3% | 89.1% | 0_1ins156;371T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1536
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgaggtcacc aagaattccc tggagaaaat ccttccacag ctgaaatgcc 121 atttcacctg gaacttattc aaggaagtca gtgtctcaag ggatctagaa gatagagtgt 181 gtaaccagat tgaattttta aacactgagt tcaaagctac aatgtacaac ttgttggcct 241 acataaaaca cctagatggt aacaacgagg cagccctgga atgcttacgg caagctgaag 301 agttaatcca gcaagaacat gctgaccaag cagaaatcag aagtctagtc acttggggaa 361 actacgcctg ggtctactat cacttgggca gactctcaga tgctcagatt tatgtagata 421 aggtgaaaca aacctgcaag aaattttcaa atccatacag tattgagtat tctgaacttg 481 actgtgagga agggtggaca caactgaagt gtggaagaaa tgaaagggcg aaggtgtgtt 541 ttgagaaggc tctggaagaa aagcccaaca acccagaatt ctcctctgga ccggcaattg 601 cgatgtacca tctggataat cacccagaga aacagttctc tactgatgtt ttgaagcagg 661 ccattgagct gagtcctgat aaccaatacg tcaaggttct cttgggcctg aaactgcaga 721 agatgaataa agaagctgaa ggagagcagt ttgttgaaga agccttggaa aagtctcctt 781 gccaaacaga tgtcctccgc agtgcagcca aattttacag aagaaaaggt gacctagaca 841 aagctattga actgtttcaa cgggtgttgg aatccacacc aaacaatggc tacctctatc 901 accagattgg gtgctgctac aaggcaaaag taagacaaat gcagaataca ggagaatctg 961 aagctagtgg aaataaagag atgattgaag cactaaagca atatgctatg gactattcga 1021 ataaagctct tgagaaggga ctgaatcctc tgaatgcata ctccgatctc gctgagttcc 1081 tggagacgga atgttatcag acaccattca ataaggaagt ccctgatgct gaaaagcaac 1141 aatcccatca gcgctactgc aaccttcaga aatataatgg gaagtctgaa gacaCTGCTG 1201 TGCAACATGG TTTAGAGGGT TTGTCCATAA GCAAAAAATC AACTGACAAG GAAGAGATCA 1261 AAGACCAACC ACAGAATGTA TCTGAAAATC TGCTTCCACA AAATGCACCA AATTATTGGT 1321 ATCTTCAAGG ATTAATTCAT AAGCAGAATG GAGATCTGCT GCAAGCAGCC AAATGTTATG 1381 AGAAGGAACT GGGCCGCCTG CTAAGGGATG CCCCTTCAGG CATAGGCAGT ATTTTCCTGT 1441 CAGCATCTGA GCTTGAGGAT GGTAGTGAGG AAATGGGCCA GGGCGCAGTC AGCTCCAGTC 1501 CCAGAGAGCT CCTCTCTAAC TCAGAGCAAC TGAACTGCCC AACTTTCTTG TACAAAGTGG 1561 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1621 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1681 ATGCACCCTT GTACCACCCC TATCGACGCG TTAAGTCgac aatcaacctc tggattacaa 1741 aatttgtgaa agatt