Construct: ORF TRCN0000478900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010528.1_s317c1
- Derived from:
- ccsbBroadEn_04564
- DNA Barcode:
- ATTGCTGCCCGTAGTTTCGGACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFAF2 (91942)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478900
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91942 | NDUFAF2 | NADH:ubiquinone oxidoreduct... | NM_174889.5 | 100% | 100% | |
| 2 | mouse | 75597 | Ndufaf2 | NADH dehydrogenase (ubiquin... | NM_001127346.1 | 84.9% | 81% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ttggtctcag gatttgttcc gcgccttgtg gagatcgctg tcaagggaag 121 tgaaGGAGCA CGTGGGCACG GACCAATTCG GGAACAAATA CTACTACATC CCGCAGTACA 181 AGAACTGGAG AGGACAAACT ATTCGAGAGA AAAGAATTGT AGAAGCAGCA AATAAAAAAG 241 AAGTAGACTA TGAAGCAGGG GATATTCCAA CAGAATGGGA AGCTTGGATT AGAAGAACAA 301 GAAAGACTCC ACCTACTATG GAGGAAATAC TAAAGAATGA AAAACACAGA GAAGAAATCA 361 AAATAAAAAG CCAAGATTTT TATGAAAAAG AAAAACTCCT TAGTAAAGAG ACCAGTGAGG 421 AACTCCTGCC TCCACCAGTT CAAACTCAAA TTAAAGGCCA TGCCTCTGCT CCATACTTTG 481 GAAAGGAAGA ACCCTCAGTG GCTCCCAGCA GCACTGGTAA AACCTTTCAG CCAGGATCCT 541 GGATGCCACG AGATGGCAAG AGCCACAATC AATGCCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 721 TGCTGCCCGT AGTTTCGGAC CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt